The p53 Mutation/Deletion Profle in a Small Cohort of the Omani Population with Diffuse Large B-Cell Lymphoma
Journal Title: Sultan Qaboos University Medical Journal - Year 2014, Vol 14, Issue 1
Abstract
Objectives: Mutations/deletions affecting the TP53 gene are considered an independent marker predicting a poor prognosis for patients with diffuse large B-cell lymphoma (DLBCL). A cohort within a genetically isolated population was investigated for p53 mutation/deletion status. Methods: Deoxyribonucleic acid (DNA) samples were extracted from 23 parafn-embedded blocks obtained from DLBCL patients, and subjected to polymerase chain reaction (PCR) amplifcation and sequencing of exons 4–9 of the p53 gene. Results: While 35% of patients analysed displayed allelic deletions (P <0.01), immunohistochemical analysis revealed a mutation rate of 69.5%. It is noteworthy that the rate of p53 mutations/deletions in this small cohort was found to be higher than that previously reported in the literature. Interestingly, patients with p53 mutations displayed a better overall survival when compared to those without. Te survival of patients treated with rituximab-containing combination chemotherapy was signifcantly better than those who did not receive rituximab (P <0.05). Furthermore, a modelling analysis of the deleted form of p53 revealed a huge structural change affecting the DNA-binding domain. Conclusion: Te TP53 mutation/deletion status plays a role in mechanism(s) ruling the pathogenesis of DLBCL and may be useful for stratifying patients into distinct prognostic subsets.
Authors and Affiliations
Yahya Tamimi| Departments of Biochemistry, College of Medicine & Health Sciences, Sultan Qaboos University, Sheikha Al-Harthy| Departments of Biochemistry, College of Medicine & Health Sciences, Sultan Qaboos University, Ibrahim Al-Haddabi| Deapartment of Pathology, Sultan Qaboos University Hospital, Muscat, Oman, Mohammed Al-Kindi| Departments of Biochemistry, College of Medicine & Health Sciences, Sultan Qaboos University, Hamza Babiker| Departments of Biochemistry, College of Medicine & Health Sciences, Sultan Qaboos University, Mansour Al-Moundhri| Departments of Medicine, College of Medicine & Health Sciences, Sultan Qaboos University, Ikram Burney| Departments of Medicine, Sultan Qaboos University Hospital, Muscat, Oman
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