THE PENETRATION TEST WITH Zona pellucida AS A PREDICTOR OF THE FERTILIZING CAPACITY OF NATIVE AND CRYOPRESERVED HUMAN SPERMATOZOA
Journal Title: Український журнал медицини, біології та спорту - Year 2017, Vol 3, Issue 1
Abstract
In order to fertilizate oocyte the sperm need to penetrate into Zona pellucida surrounding the female gamete and consisting of glycoprotein. The purpose of this work was to evaluate the penetration activity of native and cryopreserved sperm with normo- and oligoastenoteratozoospermia. Control group 1 consisted of 10 samples of ejaculates with normozoospermia. Group 2 included the samples of 22 men with oligoasthenoteratozoospermia. Cryopreservation of spermatozoa was carried out by vitrification. The penetration rate and index were calculated by counting the number of spermatozoa associated with empty ZP oocytes. The average number of spermatozoa in group 1 was 46.7 ± 8.8, in group 2 it made 12.4 ± 3.6 million/ml (p<0.01). The fractions of progressive motile spermatozoa were 27.9 ± 3.5 and 8.2 ± 1.1% (p<0.001), for group 1 and 2, respectively. We found significant differences in the ability of spermatozoa from group 1 and 2 to penetrate ZP. The penetration rate of spermatozoa with normozoospermia was 86.6 ± 8.5%, with oligoasthenoteratozoospermia it made 17.9 ± 4.5% (p <0.001). After cryopreservation, the penetration rate of spermatozoa with normozoospermia did not change and made 84.4 ± 9.2% (р<0.05), however, with oligoasthenoteratozoospermia was significantly decreased down to 9.9 ± 3.3% (р <0.001). The penetration index was 0.85 ± 0.19 and 0.17 ± 0.07% for groups 1 and 2, respectively (p <0.01). After cryopreservation, the penetration index for spermatozoa from normozoospermic ejaculates remained at the same level, whereas in oligoastenoteratozoospermia was decreased up to 0.03 ± 0.06% (p<0.01). ZP penetration rate and index of spermatozoa from normozoospermic ejaculates is significantly higher than with oligoastenoteratozoospermia. Cryopreservation factors do not affect to spermatozoa ability to bind with ZP in normozoospermic group and significantly reduce this index at oligoasthenoteratozoospermic group. The penetration test with Zona pellucida can be used as a predictor of fertilizing ability of native and cryopreserved human spermatozoa. These results should be considered when choosing the tactics of fertilization with cryopreserved spermatozoa, especially in the case of oligoasthenoteratozoospermic semen samples. Promising is the elucidation of the correlation between the frequency and the index of penetration and fertilizing capacity of spermatozoa, that will be the subject of further studies.
Authors and Affiliations
M. Petrushko, T. Yurchuk, V. Pinaev, E. Pavlovich, A. Gapon
Keywords
Related Articles
- EP ID EP262945
- DOI -
- Views 107
- Downloads 0
РІВЕНЬ ЕКСКРЕЦІЇ МЕТАБОЛІТІВ СПОЛУЧНОЇ ТКАНИНИ У ПАЦІЄНТІВ РІЗНОГО ВІКУ ІЗ ОСТЕОХОНДРОЗОМ ПОПЕРЕКОВОГО ВІДДІЛУ ХРЕБТА
У статті було розглянуто питання екскреції метаболітів кісткової та хрящової тканини із сечею пацієнтів різного віку, хворих на остеохондроз поперекового відділу хребта. Було обстежено 28 пацієнтів із остеохондрозом попе...
A.viridans Autosymbionts Influence on the Piglets’ Health
Nowadays the use of personificated treatment with autostrains of probiotic bacteria becomes more and more popular. One of the most perspective microorganisms that can be used as a basis for creation of such therapy is A....
The Distribution of Genotypes of Polymorphism A1166c of the Angiotensin Ii Type 1 Receptor Gene in Patients with Arterial Hypertension in Combination with Non-Alcohol Fatty Liver Disease
The aim of the study is to estimate the distribution of genotypes of polymorphism A1166C of the angiotensin II type 1 receptor gene in patients with arterial hypertension and non-alcoholic fatty liver disease. Materials...
АНАЛІЗ ЗАХВОРЮВАНОСТІ СЕРЕД ПРАЦІВНИКІВ, ЯКІ ВИКОНУЮТЬ РОБОТИ ПІДВИЩЕНОЇ НЕБЕЗПЕКИ
Метою роботи було проведення аналізу захворюваності серед працівників, які виконують роботи підвищеної небезпеки. Медичні огляди проводились згідно Наказу МОЗ України № 246 від 21.05.2007 року «Про затвердження Порядку п...
Evaluation of Myocardium Contractile Function and Systemic Inflammatory Response Indices in Hypertensive Disease in Combination with Chronic Obstructive Pulmonary Disease in the Process of Treatment
Pathogenic connections of hypertension and chronic obstructive pulmonary disease (COPD) are poorly understood. According to several studies in patients with COPD, the cardiovascular causes of death are about 25%. The fun...