The possible role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 at the leading and trailing edges of the breast cancer cell line
Journal Title: Iberoamerican Journal of Medicine - Year 2021, Vol 3, Issue 1
Abstract
Introduction: Phosphoinositides play a key role in the regulation of focal adhesions (FAs) turnover during cell adhesion and migration. However, their potential role in FA turnover at leading and trailing edge of cell are not yet fully understood. In this study, we investigate their spatial co-localisation with paxillin directly at leading and trailing edge of MDA-MB-231 breast cancer cell line. Materials and methods: Cell lines and cell culture experiments were done using MDA-MB-231human adenocarcinoma cells. Co-trnasfection and confocal microscopy were performed to visualise phosphoinositides and FAs by using GFP-C1-PLCdelta-PH/Btk-PH-GFP and 3 μɡ paxillin-RFP as biosensors. Then, ImageJ was used to measure co-localisation point between Phosphatidylinositol 4,5-trisphosphate (PtdIns(4,5)P2) or Phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) and paxillin Spearman's rank correlation value was taken between PtdIns(4,5)P2/PtdIns(3,4,5)P3. Results: Our results demonstrate that the spatial co-localistion of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 with FA at leading and trailing age of cell were slightly changed. Conclusions: This suggests that PtdIns(4,5)P2 and PtdIns(3,4,5)P3 play an equal role at the leading and trailing edges of the cancer metastasis through interaction with FA proteins.
Authors and Affiliations
Dhurgam AL-Fahad
A rare case of Amyand’s hernia with acute appendicitis in a 69-year-old woman: a case report
Introduction: Amyand’s hernia is an extremely rare and atypical hernia that is difficult to diagnose clinically characterized by the herniation of the appendix into the inguinal sac. The aim of this report is to describe...
A Systematic Review of the Calcium Content of the Normal Human Prostate Gland
Introduction: There is much lack of knowledge concerning most prostatic malfunction, especially the reasons and detailed nature of its pathologies. In spite of advances in medical science, the differential diagnosis of p...
Treatment outcomes and influencing factors on patients with Pulmonary Tuberculosis: a retrospective study
Introduction: Tuberculosis infection caused by Mycobacterium tuberculosis is one of the most common infectious diseases, especially in countries such as Iran. The course of treatment and the number of drugs used vary dep...
Critical Care Nursing
Everyday routine jobs, as well as suddenly specific situations, as well as severe medical conditions of a nurse, can be considerably psychophysically exhausted. It is therefore important for nurses to find a model to dea...
Incidence of Bacterial Skin Infections in Libya: A Retrospective Population-Based Study
Introduction: The global burden of bacterial skin infection is substantial. We aimed to determine the common pathogens causes skin infections and their antimicrobial resistance pattern. Methods: A retrospective record r...