The Protective Effect of Coenzyme Q10 on the Renal Ischemia Reperfusion Induced Liver Injury
Journal Title: Ankara Üniversitesi Tıp Fakültesi Mecmuası - Year 2019, Vol 72, Issue 2
Abstract
Objectives: The aim of this study was to show the efficacy of coenzyme Q10, known as anti-oxidant with anti-inflammatory properties, in liver damage after renal ischemia-reperfusion (RIR). Materials and Methods: For this purpose, rats were divided into three groups to design an experimental RIR model. Group 1 rats were Sham group. Group 2 rats underwent RIR without any medication. Group 3 rats were given coenzyme Q10 before RIR. In order to evaluate the damage, serum urea, creatinine, aspartate aminotransferase (AST) and alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and gamma glutamyl transferase (GGT) levels were investigated and additionally tissue (MPO), malondialdehyde (MDA), total sulfhydryl (SH), total nitrite and myeloperoxidase (MPO) levels were investigated. Histopathologic examination was performed for all groups and DNA fragmentation resulting from apoptotic fragmentation was evaluated with caspase-3 and TUNEL with immunohistochemical methods. Results: Urea and creatinine levels and also ALP, ALT, AST, GGT and LDH levels were significantly increased in group 2 compared to the Sham group. These increases were significantly lower in group 3. The tissue levels of MDA, nitrite, and MPO were significantly increased in group 2 compared to the Sham group, and SH levels were significantly decreased in group 2. In the group 3 rats, MDA, nitrite and MPO levels were decreased when compared to group 2. Additionally there was a significant increase in histopathologic scores in group 2 compared to the Sham group, whereas the decrease in histopathologic scores of group 3 was found to be significant when compared to group 2. Similarly, caspase-3 and TUNEL scores were significantly high in group 2 compared to Sham group and the scores were significantly low in group 3 compared to group 2. Conclusion: Coenzyme Q10 improved the histopathological findings, the serum tests and tissue oxidative stress levels in post-RIR liver tissues. Our results show that coenzyme Q10 can play an important role in protection of liver against RIR-induced damage.
Authors and Affiliations
Aylin Akbulut, Buğra Bilge Keseroğlu, Gökhan Koca, Cem Nedim Yücetürk, Berat Cem Özgür, Hatice Sürer, Elmas Öğüş, Nihat Yumuşak, Jale Karakaya, Meliha Korkmaz
Keywords
Related Articles
- EP ID EP671750
- DOI 10.4274/atfm.galenos.2019.46338
- Views 205
- Downloads 0
Evaluation of Time and Color Perception in Children and Adults
Objectives: The study aimed to compare the time and color perception among adults and children to reveal the age-dependent change of the time perception. Materials and Methods: The children (n=30; age: 7-12, 50% female)...
Hemispheric Differences in Brain Diffusion Values in 3 Tesla MR
Objectives: The purpose of this study is to demonstrate the variability of the diffusion values in different anatomical regions of the brain. Materials and Methods: 3T diffusion MR images of 190 patients underwent brain...
Factors Affecting Prognosis in Hypoxic Encephalopathy Patients Followed-up in Intensive Care Unit
Objectives: Hypoxic encephalopathy (HE) after cardiac arrest is a clinical condition that can cause severe neurological sequelae and death. It is important to determine the prognosis in patients with HE for follow up and...
Acute Mesenteric Ischemia and Splenic Infarct After Coronary Bypass Surgery: An Analysis of 32 Patients
Objectives: Acute mesenteric ischemia and other end organ emboli are serious life-threatening complications after open-heart surgery. In this study, we evaluated the outcome of patients with mesenteric ischemia and splen...
Comparasion Between Mitotic Activity, Ki-67 Index and Platelet Volume Index as a Prognostic Marker in Gliablastomas
Objectives: An elevated platelet count is considered an independent prediction of survey in glioblastoma and various other intracranial entities. Activity of the tumor microcirculation resulting in platelet activation an...