The protective effect of vitamin C on phenylhydrazine-induced hemolytic anemia on sperm quality and in-vitro embryo development in mice
Journal Title: International Journal of Reproductive BioMedicine - Year 2018, Vol 16, Issue 12
Abstract
Background: Phenylhydrazine (PHZ) induced anemia and was shown to have harmful effects on the male reproductive system. Objective: To investigate the protective effect of vitamin C (Vit C) on sperm parameters quality, in vitro fertilization potential and embryonic development in a mouse model of hemolytic anemia induced by PHZ. Materials and Methods: Thirty-two NMRI adult male mice (n = 8/each) were randomly classified into four groups. Group I (control) received normal saline, Group II (PHZ) received 8 mg/100 gr body weight PHZ as initial dose, continued by 6 mg/100 gr intraperitoneally every 48 hr, Group III (Vit C) received Vit C (10 mg/kg, daily, intraperitoneally), and group IV (PHZ + Vit C) received PHZ and Vit C. After 35 days, sperm quality parameters, the percentage of sperm with DNA damage and in vitro fertilization outcomes up to blastocyst stage were evaluated. Results: A significant (p < 0.001) reduction in all of the sperm parameters (count, motility, viability and normal morphology) were observed in group II (PHZ) compared with group I (control). In group IV (PHZ ± Vit C), these parameters and sperm DNA damage (p < 0.001) improved significantly when compared with PHZ-treated mice. Furthermore, PHZ caused a significant (p < 0.001) decrease in the fertilization rate and the percentage of pre-implantation embryos’ (two cell embryo and blastocyst) formation in comparison to group I (control), and Vit C supplementation in mice of group IV improved significantly the fertilization rate (p = 0.002), but it could not improve the percentage of two cell embryos and blastocyst production. Conclusion: The data from this study indicated that Vit C decreased the adverse effects of PHZ on the quality of sperm parameters and in vitro fertilization rate, but it is insufficient to restore the in-vitro embryonic development and fertility potential.
Authors and Affiliations
Mojtaba Karimipour, Zahra Dibayi, Abass Ahmadi, Masoumeh Zirak Javanmard, Elnaz Hosseinalipour
Keywords
Related Articles
- EP ID EP642609
- DOI -
- Views 80
- Downloads 0
The effect of Myo-inositol on sperm parameters and pregnancy rate in oligoasthenospermic men treated with IUI: A randomized clinical trial
Background: In about 40% of the couples, the cause of infertility problems is attributed to men because of low sperm production and disturbed motility of sperm. Pieces of evidence show that Myo-inositol has a potential r...
Comparison of sexual activity and life satisfaction in women with intended and unintended pregnancies
Pregnancy and childbirth, as a part of the triple crises of life, such as puberty and marriage, are of considerable importance and could be matter of research. Like every other crisis, pregnancy is consisted of both phys...
Complications in neonates of mothers with gestational diabetes mellitus receiving insulin therapy versus dietary regimen
Background: Gestational diabetes mellitus (GDM) is a common obstetrical complication with both maternal and fetal side effects. Objective: This study was performed to determine the complications in neonates of mothers wi...
Prevalence of polycystic ovary syndrome and its associated complications in Iranian women: A meta-analysis
Background: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age and is the most common cause of infertility due to anovulation. There is no single criterion for the diagnos...
Chlamydia antibody testing helps in identifying females with possible tubal factor infertility
Introduction: Chlamydia is an important cause of sexually transmitted diseases leading to tubal factor infertility. Background: This study aims to define the role of chlamydial antibody detection in predicting presence,...