The protein expression changes during the signaling systems interaction in stressed pea seedlings
Journal Title: Фактори експериментальної еволюції організмів - Year 2018, Vol 22, Issue
Abstract
Aim. The plant’s signaling systems functioning under stress impact is expressed in changes of the genes expression and protein synthesis. The purpose of this study is to investigate the effect of stressors and their combinations on pea seedlings at the molecular level, qualitative and quantitative changes in the spectrum of plant proteins. Methods. This phenomenon were investigated on four experimental groups: control seedlings, plants irradiated with gamma rays in the dose of 10 Gy, pea exposed to salt stress (0.22 Mol/L NaCl solution) and both stressors consequently. Proteins were isolated from each group and analyzed using ultra-high performance liquid chromatography. Results and conclusions. We observed the modification of expression of eight identified proteins: transketolase, malate dehydrogenase (43.52 kDa and 41.98 kDa), translation elongation factor EF-2 subunit, 14-3-3-like protein, heat shock cognate protein 80, heat shock cognate 70 kDa-like, 14-3-3-like protein B. Their significant role in the stress signals transduction and in the processes of forming an active response to the adverse factors is confirmed by concentration fluctuations between groups. The largest number of proteins has changed in response to the combined effect of ionizing radiation and salinity. Each factor by itself cause changes in less quantity of proteins.Keywords: signaling systems, proteins, pea seedlings, stress factors.
Authors and Affiliations
О. Г. Нестеренко, С. В. Літвінов, Н. М. Рашидов
Keywords
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