The proteome response of barley root (Hordeum vulgare L.) to cold stress
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 7, Issue 6
Abstract
Low temperature limits distribution and productivity of plants, and causes genetic, morphologic and physiologic changes. The aim of this research is to investigate the cold stress effect of 4°C on morphological traits and proteome profile of root in a cold tolerant barley cultivar (EC83-1215). For this purpose, the seeds were grown in greenhouse, and the cold stress was imposed at seedling stage. The root morphological characteristics and proteome profile were examined, after 48 hours of imposing cold stress and compared with plants grown under 25°C as control. Two-dimensional electrophoresis (2-DE) was employed for proteomic analysis and total protein was extracted using TCA-acetone method. The first dimension of the electrophoresis performed as iso-electric focusing in tube gels and the second dimension carried out using slab sodium dodecyl sulfate polyacrylamide gels. Examination of the 2-DE gels showed that among 72 repeatable protein spots, 15 proteins indicated significant changes in which, 10 and 5 protein spot were up-and down-regulated, respectively. Statistical analysis revealed that applying 4°C cold stress on plants did not change the root related traits including number of roots, root fresh weight, root volume, root surface and root diameter, statistically. Therefore, lower temperatures may be needed if we wish to see any changes in these traits.
Authors and Affiliations
Mahta Mohamadiaza, Mahmoud Toorchi
Keywords
Related Articles
- EP ID EP38688
- DOI -
- Views 196
- Downloads 0
Floristic composition, life form and leaf size spectra of the coal mine area vegetation of darra adam khel, khyber pakhtonkhwa, Pakistan
An Inventory of plant species of Coal Mine area of Darra Adam Khel was prepared on the basis of field trips conducted in different parts of the year 2009, particularly in winter, summer and monsoon. The flora consisted...
Predation potential of foliage spiders and estimates of utilization curve, niche breadth and overlap in cotton filed from Punjab, Pakistan
Production of cotton is restricted by pest infestation causing deterioration in lint quality. Spiders being generalist predators regulate insect populations. Bio diversity and relative abundance of spiders as predator a...
Effect of salicylic acid on the expression of a gene involved in the biosynthesis of hyperforin in St. John’s wort (Hypericum perforatum)
To address the role of the gene HpPKS1 in the biosynthesis of hyperforin in St. John’s wort, we analyzed the effect of different concentrations of salicylic acid on the expression of this gene via qRT-PCR method. To do t...
Change in the population dynamics of spiders with the seasonal variations in the Sugarcane fields
In the present study, spider diversity and seasonal variation in the population dynamics was studied. A total of 192 specimens were recorded from the sugarcane fields located in the district Faisalabad (habitat-1). These...
Arsenic concentrations in commercial fish from freshwater and saltwater
Research was performed to investigate the concentration of arsenic in muscle and liver of fish species from freshwater, Barbus luteus, Aspius vorax, Cyprinion macrostomus in Karoon Rivers and saltwater, Liza dussumieri...