The regulated-up of expression of type Ⅲ voltage-gated sodium channel ⅢN in spontaneously epileptic rat brain hippocampus
Journal Title: Science Paper Online - Year 2006, Vol 1, Issue 4
Abstract
[Objective] To compare the expression of coding voltage dependent Ⅰ,Ⅱ and Ⅲ sodium channel α-isoform in spontaneously epileptic rats (SER) and control rats. [Methods] Total RNA is extracted from occipital cortex, dentate gyrus, CA1 and CA3 hippocampus, all types sodium channel are observed using PCR A protocol after reverse transcription, and the expression of typeⅠ, ⅡA, ⅡN, Ⅲ and ⅢA, ⅢN sodium channels is respectively determined after restricted digestion of PCR B, C and D products. Type ⅢN sodium channel protein expression is detected by immunofluoresence. [Results] In spontaneously epileptic rats, all types of sodium channel expresse in a higher level than those of control groups in occipital cortex, dentate gyrus ,CA1 and CA3 hippocampus without significant difference (P＞0.05). Relative proportion of sodium channel Ⅰ, Ⅱ, Ⅱ and Ⅲ mRNAs in four adult brain areas from control rats and SER have no significant difference (P＞0.05). But type Ⅲ sodium channel expresses higher than that in control groups in hippocampus, restriction mapping analysis shows that ⅢN increases significantly (P＜0.01). [Conclusion] The expression of type Ⅲ sodium channel ⅢN is up regulated in hippocampus of spontaneously epileptic rat brain.
Authors and Affiliations
Na Yu , Feng Guo , Wa Du
Keywords
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