The relationship among mitochondrial dysfunction and antibiotic activities on Toxoplasma gondii in vitro
Journal Title: World Journal of Pharmaceutical Sciences - Year 2017, Vol 5, Issue 9
Abstract
Toxoplasma gondii is the causative agent of toxoplasmosis, which current chemotherapies are self-limiting due to inability to eliminate the parasite and induce toxic effects. Then, new therapies are necessary and antibiotics in combinations with new drugs can be an alternative. Camptothecin, ciprofloxacin and tetracycline, known by inducing loss of mitochondrial activity, were used to verify their anti-T. gondii activities. Infected host cells were incubated with the antibiotics at different concentrations (1-100 M) for 12 and 24 h. The parasites were eliminated from 5 M, 12 h, with no toxic effect to host cell until 50 M, 24 h. The reversibility assays confirmed that the camptothecin and tetracycline arrested the parasite replicative cycle, but not ciprofloxacin. The mitochondrial activity of parasite were gradually decreased during antibiotic treatments, although no morphological change was observed. In summary, the intracellular tachyzoites were eliminated from intracellular environment in presence of the antibiotics, possibly due to loss of its mitochondrial activity.
Authors and Affiliations
Laís Pessanha de Carvalho
Keywords
Related Articles
- EP ID EP331130
- DOI -
- Views 101
- Downloads 0
Antibiotic susceptibility of Streptococcus pyogenes and Staphylococcus aureus isolated from Pharyngitis and Tonsillitis patients in Nasiriyah City, Iraq
The present study aimed to isolation and identification and antibiotic susceptibility test for Streptococcus pyogenes and Staphylococcus aureus bacteria isolated from Pharyngitis and Tonsillitis patients. 276 swabs were...
In vitro antioxidant potential of secondary and tertiary growth forms of Salvinia molesta Mitchell
The aquatic weed Salvinia molesta causes adverse ecological disruptions leading to considerable social, ecological and economic damages. It reduces dissolved oxygen content, blocks sunlight reaching the other aquatic spe...
Actomyosin filaments and ATP - heart of rigor mortis
It is a well-known fact that contractile substance of the muscle fibril is actomyosin, a complex composed of two protein filaments- actin (thin filament) and myosin (Thick filament), which require adenosine triphosphate...
Study of atracurium and tramadol or ketorolac as an adjunct to low dose lignocaine in intravenous regional anesthesia
Background: Purpose of the study was to evaluate the efficacy of Atracurium as adjuvant with tramadol or Ketorolac in reducing the dose of lignocaine used for intravenous regional anesthesia thereby decreasing the chance...
Molecular characterization and evaluation of Actinomycetes from higher altitudes of Kashmir - India
The present study was designed to explore the potent actinomycete strains with remarkable antimicrobial and cytotoxic activities from the high altitude sites of Kashmir – India. A total of 33 actinomycetes were isolated...