The Role of N-Acetylcysteine Supplementation on the Oxidative Stress Levels, Genotoxicity and Lineage Commitment Potential of Ex Vivo Murine Haematopoietic Stem/Progenitor Cells
Journal Title: Sultan Qaboos University Medical Journal - Year 2018, Vol 18, Issue 2
Abstract
Objectives: Te ex vivo maintenance of haematopoietic stem/progenitor cells (HSPCs) is crucial to ensure a sufcient supply of functional cells for research or therapeutic applications. However, when exposed to reactive oxygen species (ROS) in a normoxic microenvironment, HSPCs exhibit genomic instability which may diminish their quantity and quality. Tis study aimed to investigate the role of N-acetylcysteine (NAC) supplementation on the oxidative stress levels, genotoxicity and lineage commitment potential of murine haematopoietic stem/progenitor cells (HSPCs). Methods: Tis study was carried out at the Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia, between June 2016 and July 2017. Bone marrow cells were isolated from nine mice and cultured in a growth medium. Various concentrations of NAC between 0.125–2 µM were added to the culture for 48 hours; these cells were then compared to non-supplemented cells harvested from the remaining three mice as the control group. A trypan blue exclusion test was performed to determine cell viability, while intracellular ROS levels and genotoxicity were determined by hydroethidine staining and comet assay, respectively. Te lineage commitment potential of erythroid, myeloid and pre-B-lymphoid progenitor cells was evaluated via colony-forming cell assay. Results: NAC supplementation at 0.25, 0.5 and 2 µM signifcantly increased cell viability (P <0.050), while intracellular ROS levels signifcantly decreased at 0.25 and 0.5 µM (P <0.050). Moreover, DNA damage was signifcantly reduced at all NAC concentrations (P <0.050). Finally, the potential lineage commitment of the cells was not signifcantly affected by NAC supplementation (P >0.050). Conclusion: Te fndings of this study indicate that NAC supplementation may potentially overcome the therapeutic limitations of ex vivo-maintained HSPCs.
Authors and Affiliations
Zariyantey A. Hamid, Hui Y. Tan, Paik W. Chow, Khairul A. W. Harto, Chin Y. Chan, Jamaludin Mohamed
Keywords
Related Articles
- EP ID EP29157
- DOI -
- Views 340
- Downloads 8
Types of Bacteria associated with Neonatal Sepsis in Al-Thawra University Hospital, Sana’a, Yemen, and their Antimicrobial Profle
Tis study was undertaken to investigate the organisms causing sepsis in the Neonatal Unit at Al-Thawra Hospital, Sana’a, Yemen, determine their resistance to antibiotics, and recommend policy for empirical treatment....
Re: Controversies in Odontogenic Tumours Review.
No Abstract
Acquired Methemoglobinaemia
Acquired methemoglobinaemia is a relatively rare condition and, therefore infrequently encountered in acute medical practice. Suspicion of the condition may be triggered when the measured PaO2 is ‘out of keeping’ w...
Te Relationship between Hysteroscopy, Endometrial Biopsy andTe Results of Transvaginal Sonography in Assessing Endometrial Polyps
Objective: Transvaginal Sonography (TVS) is being used in the investigation of postmenopausal bleeding (PMB). Te bleeding could be the result of a number of factors; endometrial polyps are one of the common findings in...
Antenatal Care Seeking Behaviour among Slum Mothers A Study of Rajshahi City Corporation, Bangladesh
Te study aimed to identify the important effects of some selected variables in antenatal care (ANC) seeking behaviour among slum mothers. Methods: Te data for the study were collected in 2006 from 700 mothers in the s...