The Role of Purified Urease from Proteus Mirabilis PMS17 in Stones Formation
Journal Title: Journal of Medical Science And clinical Research - Year 2015, Vol 3, Issue 8
Abstract
Introduction: Injuries and Pyelonephritis have attention of researches in the medical field, because it represents a problem of the important problems, it’s also one of the important disease frequent occurrences in human. The ability of P.mirabilis (Proteus mirabilis) PMS17 to cause these infections due to the possession of many virulence factors such as urease, hemolytic, outer membrane protein, in addition to its ability to invade cells and adhesion by pili. Material and Methods: This study was included the isolation of 67 isolates of Proteus spp. From 350 samples of urinary tract infection (U.T.I) patients from different hospitals in Baghdad city, purification of Proteus urease by three steps which included , precipitation by ammonium sulfate 25-50% saturation , ion exchange and gel filtration chromatogrophies and study behavior of the resistance stone formation in animals . Results: Results indicated that 190 samples (54, 28%) were positive results of bacteriological culture. Proteus mirabilis urease of local isolate number 17 was extracted and purified 167.35 fold with yield of 19.72% and specific activity 441.80 units / mg protein by DEAE-Sepharose and Sephacryl S-200 chromatographies. The result indicated persistent of Proteus infection in bladder and kidney of preimmunized and nonimmunized rabbits. No infection with Proteus was recorded in bladder and kidney of the flurofamide treated animals. All the preimmunized and non-immunized infected animals were showed with alkaline urine, whereas the flurofamide treated animals were showed acidurea. Urinary bladder and kidney stones were found in 50 % and 40 % respectively of the infected non-immunized animals, whereas in infected immunized animals these stones are found 40 % and 30 % respectively. Urinary stones were composed mainly of struvite and a small amount of apatite carbonate and few mixed stones.
Authors and Affiliations
Montaha A. AL-Safar
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