The stage of murine oocyte maturation correlates with their electric conductivity
Journal Title: Біологія тварин - Year 2015, Vol 17, Issue 1
Abstract
Improvement of assisted reproductive technologies is connected with a search of additional criteria and technique for gametes quality estimation and determination. Use of domestic and laboratory animals as scientific models for the females infertility overcoming is good alternative to BioMedicine. It is well-known that oocytes morphology is important criteria for further embryo development as well as for the future state of health of newborn offspring. The aim of the work is determination of electric conductivity (EC) for murine oocytes at various maturation stages retrieved from females after hormone stimulation. The EC of murine oocytes has been determined by method of electric conductivity measurement (conductometry). It has been shown that oocyte population is heterogeneous by maturation degree due to hormonal oestus induction causing the ovulation of the follicles at the different stages of growth. At the measurement of common electric conductivity of oocytes harvested from females after hormone induction of oestrus, a substantial amplitude oscillation at the resistance of different oocytes to an electric impulse been revealed. The gametes that classified as similar by maturation stage assessed as those that had the same EC. For oocytes with compact cumulus the measurement lies in the range from 27.4±10.9 to 77.9±29.3 µS/cm. The EC is significantly lower with probability (P<0.05) for oocytes in metaphase II and varies from 13.4±3.74 to 19.4±6.31 µS/cm. The oocytes EC reflects gametes’ functional prosperity and maturation degree. A high accuracy of electric impulse parameter setting and a capability to measure biological micro objects allow to consider the method of impulse electronic conductometry as a promising experimental approach to express-estimation of female gametes prosperity as well as the electroporation protocol use in assisted reproductive technologies.
Authors and Affiliations
E. I. Smolyaninova, V. A. Shigimaga, A. O. Kolesnikova, O. A. Strikha, L. I. Popivnenko, E. A. Gordienko
Keywords
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