The Status of Vitamin D and Its Receptor Fok I Gene Polymorphism Among Adult TB Patients of Mulago Hospital Kampala Uganda
Journal Title: Clinical Biotechnology and Microbiology - Year 2017, Vol 1, Issue 1
Abstract
Tuberculosis (TB) is an infectious disease causing severe problems throughout the world. However this disease is prevalent in Africa. The antimicrobial activity of vitamin D may plays a significant role in prevention of TB. The vitamin D receptor gene is associated with susceptibility. The functional Single Nucleotide Polymorphism rs2228570, Fok I gene has been found to be inconsistent among the TB patients. Additionally, patients with active TB have significantly lower vitamin D serum concentration than their contacts from the same ethnicity. This study investigated the relationship between vitamin D status and Vitamin D receptor Fok I gene polymorphism, in TB patients. A pilot case-control study was conducted in 41 newly diagnosed Tuberculosis patients and 41 non-TB healthy workers enrolled between April and June, 2013 at Mulago National Referral Hospital. Levels of Vitamin D and PTH were analyzed by Electrochemiluminescence using Cobas 6000. Genotyping of Fok I gene was done by Polymerase Chain Reaction and direct sequencing method using the ABI Sequencer. The Prevalence of hypovitaminosis in the TB patients was 24.4% and 26.8% in the healthy subjects. Vitamin D deficiency in TB patients was 9.7% and 4.9 in healthy subjects respectively. Severe vitamin D deficiency was only found in the TB patients. Subjects with vitamin D deficiency were only found in the FF genotype. There was no significant difference between vitamin D levels among the Fok I gene variants (p = 0.78). Although there was no significant difference between vitamin D status in the different genotypes of Vitamin D receptor Fok I gene polymorphism among TB patients and controls in this Ugandan cohort, the hypovitaminosis D noted dominantly in the FF genotype should not be under estimated. On the other hand optimal levels of vitamin D were predominantly found in both groups.
Authors and Affiliations
Acen L Ester, Worodria William, Mulamba A Peter, Erume Joseph
Keywords
Related Articles
- EP ID EP447933
- DOI -
- Views 66
- Downloads 0
The Status of Vitamin D and Its Receptor Fok I Gene Polymorphism Among Adult TB Patients of Mulago Hospital Kampala Uganda
Tuberculosis (TB) is an infectious disease causing severe problems throughout the world. However this disease is prevalent in Africa. The antimicrobial activity of vitamin D may plays a significant role in prevention of...