The study of antileishmanial effect of Medicago lupulina leaves essential oil on Leishmania major (MRHO/IR/75/ER) by MTT assay
Journal Title: International Journal of Farming and Allied Sciences - Year 2016, Vol 5, Issue 6
Abstract
Background and objective: Leishmaniasis is caused by protozoa of Leishmania genus and can be considered as a Zoonosis. The desease has caused the world health problems with high endemicity in developing countries such as Iran. Various chemical drugs have been used for leishmaniasis treatment, but their side effects and drug resistance haveled to look for new effective compounds. Medicago lupulina, the traditional and medicinal herb- are one valuable source of new Pharmaceutical agents. Material and Methods: The essential oil was prepared through Distillation with wather method and dried, Then solved in DMSO 5%. Leishmania major promastigotes were cultured in 2±25 °C temperature in the Schneider's medium, then in the stationary phase of RPMI-1640 culture growth medium, enriched with 10% fetal calf serum and Penicillin-Streptomycin to provide large quantity of them. Then the biological activity of essential oil was evaluated on Leishmania major promastigotes compared to glucantime drug using MTT colorometry. The optical density was measured with Eliza reader set, and the IC50 value was calculated. All tests repeated 3 times. Resalts and Discussion: Glucantime IC50 for standard parasite was equal to 12 μg/ml after 48 hours respectively. After 48 hours, IC50 for essential oil were equal to 250 μg/ml against standard parasite promastigotes respectively. Although the glucantime pharmaceutical drug was more efficient compared to investigate essential oil, the essential oil also had significantly effects on leishmania major promastigotes with higher density. Conclusion: Regarding that the studied essential oil had considerable antileishmanial effect compared to glucantime in vitro, the necessity of conducting more experiments to investigate its effect on the parasite in animal model is also appreciated.
Authors and Affiliations
Elham Gharirvand Eskandari and Monir Doudi
Keywords
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