THE STUDY OF POTENTIALITY OF PICRORHIZA KURROA ROOT PROTEINS TO INHIBIT FREE RADICALS AND Α-AMYLASE ENZYME
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2015, Vol 8, Issue 2
Abstract
 Objective: To prevent oxidative damage to cells caused by free radicals that are responsible for many diseases, such as atherosclerosis, diabetesand cancer. Picrorhiza kurroa (Katuki) is an ayurvedic medicinal plant used in the current study to evaluate the effectiveness of its root proteinsprecipitated at 60% ammonium sulfate, and further dialyzed protein sample was investigated for scavenging free radicals and also inhibiting alphaamylasewhich helps to control postprandial hyperglycemia.Methods: The present study was done by Anti-oxidant methods 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, hydroxyl scavenging method, nitricoxide (NO) method, total anti-oxidant method , α-amylase inhibition and its kinetic studies by DNS method.Results: The protein significantly inhibited DPPH radicals 66.19±6.08%, hydroxyl radicals 59.18±9.05%, nitric oxide radicals 45.38±19.52% andtotal antioxidants present is 82.91±2.81% compared with standard ascorbic acid 93.67±3.91%. The protein extract (60%) inhibited rat pancreaticα-amylase 41.62±22.3% compared with acarbose standard 91.09±1.8%. These results provide valuable information that P. kurroa proteins exhibitsgood antioxidant and anti-diabetic property.Conclusion: Picrorhiza kurroa root proteins (60%) posses potent antioxidant and antidiabetic effects. Kinetic studies showed it is a non competitiveinhibition. alpha-amylase inhibitor is a protein which inhibits rat pancreatic alpha-amylase specifically at a optimum temperature 37°c, pH 6.9, andtime 35’.Keywords: Picrorhiza kurroa, Free radicals, Anti-oxidant, Anti-diabetic, α-amylase.
Authors and Affiliations
Sanjay S, Syeda Hajira Banu, Chethankumar M
Keywords
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