The study of the influence of temperature, nutrient medium and acetate buffer addition on glucose fermentation process
Journal Title: Annals of WULS, Forestry and Wood Technology; Zeszyty naukowe SGGW - Year 2015, Vol 91, Issue
Abstract
The study of the influence of temperature, nutrient medium and acetate buffer addition on glucose fermentation process. In this paper fermentation process of glucose in various conditions was studied. The aim of the studies was to examine the influence of various factors (temperature, nutrient medium and acetate buffer addition) on glucose fermentation process. In the studies, standard of anhydrous D-glucose was used as a research material. The glucose standard solutions and fermentation mixtures were analyzed by the HPLC with a refractometric detector. Based on the studies, it was found that 0.1% of diammonium hydrogen phosphate addition as a nutrient medium has a positive effect on ethanol production. On the other hand, the acetate buffer (pH = 5) addition has a negative effect. Additionally, it was confirmed that the temperature of fermentation process is a very important factor, which affects the yield of ethanol. After 23 days at 30 °C the production of ethyl alcohol occurs with about twice higher yield than at 25 °C
Authors and Affiliations
Andrzej Antczak, NATALIA SPYSZEWSKA, Janusz Zawadzki, Monika Marchwicka, Adam Kupczyk
Keywords
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