The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study
Journal Title: International Journal of Reproductive BioMedicine - Year 2019, Vol 17, Issue 4
Abstract
Background: Cyclophosphamide (CP) is a well-known alkylating anticancer agent used in the treatment of various malignant and non-malignant tumors. CP may also cause a variety of adverse effects, including reproductive toxicity. Amifostine is known as a cytoprotective drug having antioxidant properties. Objective: To evaluate the possible beneficial effects of amifostine on testicular toxicity induced by CP in rats. Materials and Methods: A total of 35 Sprague-Dawley rats were used in this experimental study. The CP group animals received a single dose of 200 mg/kg CP on Day 8 by intraperitoneal injection and were left untreated for the following seven days. The two remaining groups of animals were treated with 200 mg/kg/day amifostine (AMF 200) and 400 mg/kg/day amifostine (AMF 400) for seven days prior to and following a single intraperitoneal injection of CP. Morphometrical analysis and histological examination of testicular tissue were performed. Serum testosterone, luteinizing hormone, and follicle-stimulating hormone levels were measured in serum using commercial ELISA kits. The epidydimal sperm count was determined. Results: The tubular epithelial height in the testis was significantly higher in the AMF400 group compared to other groups (p < 0.001). Animals in the AMF400 group showed minimal debris in the tubules, no Sertoli cell damage, and the Johnsen scores were slightly higher in the AMF400 group. The epididymal sperm count was significantly lower in the CP-administered animals compared to the control animals and was significantly higher in the AMF200 and AMF400 groups compared to the CP group (p = 0.006, and p = 0.019 respectively). Conclusion: Amifostine, at a dose of 400 mg/kg, may have a protective effect on testicular damage induced by CP in rats.
Authors and Affiliations
Coskun Kaya, Ali Barbaros Baseskioglu, Semra Yigitaslan, Fikriye Yasemin Ozatik, Orhan Ozatik, Sema Uslu
Keywords
Related Articles
- EP ID EP642619
- DOI -
- Views 97
- Downloads 0
The prevalence of polycystic ovary syndrome in adolescents: A systematic review and meta-analysis
Background: Polycystic ovarian syndrome is an endocrine disorder with many complications. This syndrome is a growing concern among adolescents around the world, with varying reports of its prevalence in different parts o...
Polymorphism of MnSOD (Val16Ala) gene in pregnancies with blighted ovum: A case-control study
Background: Blighted ovum is one of the most common reasons for abortion during the first three months of pregnancy. Manganese superoxide dismutase (MnSOD) is an important antioxidant enzyme in the human immune system. T...
Effect of antioxidant therapy on the sperm DNA integrity improvement; a longitudinal cohort study
Background: The effect of antioxidant therapy on sperm DNA fragmentation index (DFI) and achieving natural pregnancy were under debate. Very few studies have showed the rate of pregnancy rate after the antioxidant therap...
Reconstruction of mammalian oocytes by germinal vesicle transfer: A systematic review
Nuclear transfer procedures have been recently applied for clinical and research targets as a novel assisted reproductive technique and were used for increasing the oocyte activity during its growth and maturation. In th...
Y chromosome microdeletions frequency in idiopathic azoospermia, oligoasthenozoospermia, and oligospermia
Background: Genetic factors are candidates for about 30% of male infertility with sperm production-related abnormalities. Y chromosome microdeletions are responsible for around 10% of male infertility. These microdeletio...