The Toxic Effects of Lead on Testicular Macrophage Immunomodualation and Sperm Cell Parameters in Mice
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2015, Vol 9, Issue 5
Abstract
Aims: In the present study the toxic effects of lead was investigated experimentally on the testicular macrophages and sperm cells isolated from testes of adult male mice to ascertain the extent of immunomodulation and reproductive dysfunctions (in-vivo). Study Design: Experimental study. Place and Duration of Study: Department of Biotechnology, Assam University, Silchar, Assam, India, between March 2013 and August 2014. Methodology: Dose response study was carried out with an increasing concentration of lead acetate. Percent mortality was determined for these doses and plotted graphically against the respective doses. From the graphs, LD50 value was determined. To validate immunomodulation of testicular macrophages and reproductive dysfunction due to lead intoxication, mice were divided into two groups. One group is treated with lead acetate (10 mg/kg body weight) and the other group with isotonic saline solution for 15 days. The isolated testicular macrophages were used to study the phagocytic property, alteration of enzyme release, cytokine release assay and the sperm cell were used for studying the sperm parameters in both control and treated group. Results: From the study significant decrease in phagocytic index (25516.61±1352.69 to 5154.67±437.37), myeloperoxidase release (77.3±10.7μM to 23.6±4.9μM), nitric oxide release (9.2±1.13 to 4.7±1.69) and a concomitant rise in the pro-inflammatory cytokine TNF-α were observed. These leads to an increased oxidative stress and inflammatory damage in the testes and subsequently less sperm count (78±1.155 to 24.33±1.764), sperm motility and abnormal sperm morphology was documented. Conclusion: Thus it could be concluded that the toxic potential of lead diminished the functional capacities of testicular macrophages, led to immunomodulation and inflammatory damage in testes and thus impede the sperm function parameters, which bear particular significance in heavy metal induced immune infertility in male.
Authors and Affiliations
Shamim Ahmed S. K. Barbhuiya, Mahuya Sengupta
Keywords
Related Articles
- EP ID EP347698
- DOI 10.9734/BJMMR/2015/17937
- Views 51
- Downloads 0
An Unpleasant Result of Imprecision: Esophageal Corrosive Injury Due to the Oral Intake of 10% Benzalkonium Chloride
Benzalkonium chloride is a skin antiseptic agent. It should be diluted before usage for cleaning of mucosal membranes; otherwise it may result in severe damage on mucosa. Herein we will report a 2 months old baby who too...
The Value of Cytology in the Diagnosis of Pleural Effusions
The aim of this study is to assess the value of cytology in the diagnosis of pleural effusions. It is a retrospective review of the patients with pleural effusions admitted at our clinic in a 8-year period. We evaluated...
Longevinex ® Improves Human Atrophic Aged-related Macular Degeneration (AMD) Photoreceptor / Retinal Pigment Epithelium Mediated Dark Adaptation
Aim: Gradual photoreceptor/ RPE deterioration/ vision loss in AMD is common, irrespective of US NEI AREDS I/II supplement risk reduction, or intra-vitreal anti-VEGF pharmacology. We evaluated dark adaptation (DA), a broa...
Support Vector Machine-based Multi-scale Entropy of Curves Recognition for Electrocardiogram Data
Objective: Multiscale entropy (MSE) analysis has been widely used to analyze the physiological signals in the frequency domain. Higher complexities of MSE curve present in the physiological system have the better ability...
Streptococcus Toxic Shock Syndrome Due to Non Haemolytic Streptococcus pyogenes
Aim: Describe the detection of an unusual pathogen associated with a common clinical presentation with abrupt worsening. Case Presentation: We present the case of a 36-year-old Peruvian male who presented to the E...