The Use of herbal Extracts of Ocimum sanctum and Mimusops elengi as a Novel Storage Media for the Avulsed Tooth
Journal Title: Journal of Advanced Medical and Dental Sciences Research - Year 2018, Vol 6, Issue 1
Abstract
Background: Maintaining the viability of PDL cells of the avulsed tooth is of utmost importance for a successful re implantation procedure. The storage medium plays a significant role in this aspect, but the immediate availability of the commercial storage medium has been a major problem. Storage media prepared with readily available herbs like Ocimum sanctum (tulasi) and Mimusops elengi (bakul) which have anti-inflammatory, anti-oxidant, anti-bacterial and anti-fungal properties would be of great help in this regard. Aim: To evaluate the efficacy of Ocimum sanctum and Mimusops elengi as storage media for avulsed teeth. Materials and Methods: The periodontal ligament cells obtained from the scrapings of orthodontically extracted teeth were stored separately in Milk, and extracts of Ocimum sanctum and Mimusops elengi for 60 minutes. They were then transferred into a falcon tube containing 2ml of growth media and incubated for 24 hours followed by suspension in phosphate buffer solution for 10 minutes. The PDL cells were then kept in 5ml of Trypsin-EDTA solution for10 minutes and centrifuged for 5 minutes at 800 rpm. The residue collected is added with 0.4% Trypan blue solution to check the viability of the PDL cells using optical microscope. Results: The results indicate that Milk could preserve the viability of 88% of PDL cells, where in Ocimum sanctum and Mimusops elengi could maintain viability of 70% and 30% cells respectively. Conclusion: In the absence of commercially available storage medium, Ocimum sanctum extracts could be recommended as potential alternatives.
Authors and Affiliations
K. Nidesh Kumar, Ch Srinivas Kumar, Vanga V Narsimha Rao
Keywords
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