THE USE OF RAPD-PCR AND PCR-RFLP MOLECULAR MARKERS TO GENETICALLY DISTINGUISH THE MORPHOLOGICALLY CLOSE TWO SPECIES OF METAPENAEUS GENUS FROM TWO DIFFERENT ENVIRONMENTAL LOCATIONS( KAUR ABDULLAH & SHATT AL ARAB ) IN WATERS SOUTHERN OF IRAQ.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2018, Vol 6, Issue 3
Abstract
RAPD-PCR and RFLP?PCR molecular markers were used to study the Genetic Characterization for two members in Metapenaeus genera of Penaeidae shrimps from two different environment southern of Iraq. Metapenaeus affinis which is in kaur Abdullah (salty water) but the second is suspected member (shrimp) is in Shatt al-Arab(freshwater) While the marine closely related organism in freshwater bodies. Unlike the marine pawn, it is small but has similar morphological characteristics like the marine counterpart. The results indicate that although Metapenaes genus share considerable external features except that the genetic heterogeneity at the DNA level was high in the two organisms. RAPD marker used 12 universal primers to detect monomorphic and polymorphic patterns to genetically distinguish differences between the two organisms whereas in RFLP marker the voucher DAAPV F7 in 16S rRNA gene was amplified using PCR technique the desired gene was digested by using the enzymes (TagI , SmI and HindIII) which produced different size and numbers of bands. Thus, the voucher DAAPV F7 in 16S rRNA gene proved to be a useful molecular marker to differentiate the studied Metapenaeus species , which makes the task easier of telling apart species that are morphologically very similar.
Authors and Affiliations
Rabeeha mankhi jebur, Israa adil fadhil.
Keywords
Related Articles
- EP ID EP287957
- DOI 10.21474/IJAR01/6885
- Views 81
- Downloads 0
THE IMPACT OF AN EDUCATIONAL PROGRAM FOR NURSES ON HOW TO MANAGEMENT PAIN IN CHILDREN IN GOVERNMENTAL PEDIATRIC HOSPITALS IN KHARTOUM STATE.
Background: An Interventional study was conducted in Governmental Pediatric Hospitals in Khartoum State. Distribution of (169) nurses in five different hospitals is as follows (15%) from Gaffer Ibnaouf, (7 %) from Abdurr...
EFFECT OF YOGA ON ATTENTION LEVEL OF ORPHAN GIRLS WITH ADHD.
Mental health issues among children living in orphanages have been rising. These children often suffer from psychiatric disorders such as Attention Deficit Hyperactivity Disorder (ADHD). ADHD is characterized by inattent...
DETECTION OF HUMAN PAPILLOMMA VIRUS AND EPSTEIN-BARR VIRUS IN SUBJECTS OF ORAL LEUKOPLAKIA BY POLYMERASE CHAIN REACTION(PCR).
Viruses such as HPV and EBV have been implicated in the etiopathogenesis of oral precancer and cancer, however the association is inconclusive. Hence, the present study aimed to determine the incidence of HPV and EBV in...
GRASSROOTS WOMEN POLITICAL PARTICIPATION AND POVERTY REDUCTION STRATEGIES IN GHANA AND NIGERIA.
The practice of representative democracy across Africa for close to two decades without interruption had not translated to reduction of the level of poverty for the world’s poorest continent. Over the last decade, there...
ORAL POLIO VACCINE REFUSAL PATTERN IN KUMBOTSO LOCAL GOVERNMENT AREA, KANO STATE, NORTHERN NIGERIA.
Poliomyelitis is a disease caused by a virus, affecting the nervous system and resulting in paralysis in survivors. It is transmitted through the faeco-oral route and may result in the death of those affected if the resp...