The use of the spin probe reduction for the eastimation of the respiratory chain activity of the cardiomyocytes and hepatocytes mitochondria of the rats under the artificial hypobiosis

Journal Title: Біологія тварин - Year 2015, Vol 17, Issue 3

Abstract

The results of researches of the activity of oxidation-reduction reactions of the respiratory chain of the hepatocytes and cardiomyocytes mitochondria in the rats by the spin probe (TEMPON) electron paramagnetic resonance (EPR) was contented. The artificial hypobiosis state (the influence of hypothermia, hypercapnea and hypoxia) in the animals was created by Bakhmet’ev-Giay-Anjus model. The animals were decapitated in the state of the artificial hypobiosis (rectal temperature of 16 °C) and in 2 and 24 hours after the termination of the influence of the artificial hypobiosis factors (rectal temperature of 37 °C as a control). As a parameter of spin probe reduction the relative changes of mean field component amplitude of probe EPR spectra depending on time were used. The values of the speed constant of the spin probe reduction in the samples of the inner mitochondrial membrane were calculated by the tangent of the angle in the coordinates (lgI, t). As it was found, the reduction of the spin probe (by the value of the reduction speed constant) in the interaction with the respiratory chain of the hepatocytes under the artificial hypobiosis is similar to the respective control for the investigated temperature range (16–37 °C). However, the reduction of the spin probe in the interaction with mitochondrial respiratory chain of the cardiomyocytes under the artificial hypobiosis increases at all experimental temperatures in comparison to the corresponding control. Perhaps under the artificial hypobiosis the activation of the complex II (succinate-CoQ-oxidoreductase) of the respiratory chain of the cardiomyocyte mitochondria is done leading to the increase of redox activity of its sites which are engaged in a reduction of the probe. The changes are of short duration, because of the investigated parameters are near to the control values in a day after the influence of the factors which cause the artificial hypobiosis state.

Authors and Affiliations

S. D. Melnytchuk, S. V. Khyzhnyak, O. A. Nardid, Ya. O. Cherkashyna, V. S. Morozova, V. Riepina, V. М. Voitsitsky

Keywords

Related Articles

Estimation of growth intensity patterns in meat and egg-laying chickens of different genetic origin

The growth rate parameters used in studies (intensity formation index, growth uniformity index, stress growth index) allowed an assessment of the growth processes in meat and egg-laying chickens with different genetic ba...

Histostructure of internal organs in female rats of first generation for different doses of germanium citrate

The structural differences of the spleen, thymus, liver and lymph nodes of female rats of first generation (F1) under the influence of various doses of nano-germanium citrate (NGeCit) have been studied. The experimental...

Influence of typological features of autonomous tone on morphometric parameters of goblet cells of chickens’ intestinal mucosa

In adult chickens cross “Iza-Braun”, which were grown in conditions of poultry farms and were selected on the basis of analogues, there are two types of autonomous tone: sympathotonic (CT) — 16 specimen and sympatho-norm...

Metabolism in organism of replacement gilts by fededing with probiotic feed “PROPIG”

There are presented the results of the use of probiotic feed supplement “PROPIG” in the feeding of replacement gilts and its effect on the functional state of the organism of animals, taking into account metabolic proces...

Effect of glucose and partial dehydration on resistance of mammalian erythrocytes to hypertonic shock

The paper is devoted to the studying the resistance of human, rabbit and rat erythrocytes to the effect of hypertonic shock when changing an initial status of cells modified with glucose and 0.4 mol/l NaCl. The hemolysis...

Download PDF file
  • EP ID EP345576
  • DOI -
  • Views 86
  • Downloads 0

How To Cite

S. D. Melnytchuk, S. V. Khyzhnyak, O. A. Nardid, Ya. O. Cherkashyna, V. S. Morozova, V. Riepina, V. М. Voitsitsky (2015). The use of the spin probe reduction for the eastimation of the respiratory chain activity of the cardiomyocytes and hepatocytes mitochondria of the rats under the artificial hypobiosis. Біологія тварин, 17(3), 97-103. https://europub.co.uk/articles/-A-345576