Abstract

Objective: H. pylori is classified as class I carcinogen by World Health Organization and is a global as it is the primary cause of gastric carcinoma. The objective of present investigation was made to investigate the protective effect of homeopathically potentized H. pylori nosode in rats infected with H. pylori. Methods: The infection was induced in the rats using a bacterial suspension of 5 x 108 CFU per ml. The various animals were treated with three potencies of H. pylori nosode (3X, 6X and 12X) and standard (Clarithromycin 25mg/kg + amoxicillin 50 mg/kg + omeprazole 20 mg/kg). A period of ten weeks followed and then the rats were sacrificed. Results: H. pylori nosode was able to stop the initiation of infection in the pretreatment group of animals. This defines and provides us with the data regarding its sphere of action. A plethora of parameters such as ulcer area, infection status, biomarkers of oxidative stress, total intracellular ROS, degree of apoptosis, TNF level and load of bacteria in the gastric tissue served as the hallmarks to prove the preventive role of H. pylori nosode in the amelioration of the infection. It is evident that homeopathic drug H. pylori nosode was able to transform the susceptibility of the animals to the induction of infection. It provides a novel avenue in the field of research in homeopathy as the results suggest that H. pylori nosode was able to initiate the infection resistive forces in the animals before being subjected to the infection of H. pylori. Conclusion: H. pylori nosode possesses potent prophylactic anti H. pylori activity in vivo.

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