Therapeutic Effects of ADU-S100 as STING Agonist and CpG ODN1826 as TLR9 Agonist in CT-26 Model of Colon Carcinoma
Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2023, Vol 15, Issue 2
Abstract
Cancer immunotherapy emerged as a novel therapeutic approach to destroy tumor cells, and it has grown toward clinical transition following successful fundamental research and clinical trials. Immunotherapy by efficacious adjuvants is critical for increasing protective immune responses against infectious diseases and cancers. STING and TLR9 agonists are interesting candidates for novel immunotherapies of cancers. In this study, the antitumoral effects of ADU-S100, as a potent STING agonist, and CpG ODN1826, as a TLR9 agonist, in single and combined forms in CT-26 colon adenocarcinoma model were evaluated. This model was induced in female BALB/c mice which were divided into five groups treated with PBS, ADU-S100 (20 and 40 µg), CpG ODN (40 µg), and ADU-S100 (20 µg)+CpG ODN (20 µg). The tumor volumes and weights of mice were measured every other day. On the 30th day, the tumor, spleen, and liver tissues of mice were isolated for histopathological assessment. Hematological analysis was performed on heart blood. Intratumoral injection of agonists induced significant tumor suppression in all treatment groups with profound effect in the combination group that received half concentration of single form. Moreover, the histopathological analysis of tumor tissues showed the presence of apoptotic and inflammatory cells and increased the number of lymphocytes in the blood samples of the treatment groups indicating the effective role of these agonists in clearing the tumor. Therefore, a such synergy of adjuvants may have an effective role in cancer immunotherapy and offer new perspectives on the combination of agonists that trigger innate immune sensors during malignancy.
Authors and Affiliations
Sare Hajiabadi,Soodeh Alidadi,Mohammad Mehdi Ghahramani Senoo,Zohreh Montakhab Farahi,Hamid Reza Farzin,Alireza Haghparast,
Keywords
Related Articles
- EP ID EP722287
- DOI -
- Views 35
- Downloads 1
Purification and biological analysis of specific antigens (ESAT6/CFP10) from Mycobacterium tuberculosis
The pathogenesis of Mycobacterium tuberculosis (Mtb) is related to its low molecular weight proteins mainly ESAT6 and CFP10 that are highly specific and potentially useful for the diagnosis of tuberculosis. This research...
Investigating the Prevalence of Mycobacterium Avium Subspecies Paratuberculosis (MAP) in Industrial Dairy herds using Ziehl-Neelsen Staining, Culture, and PCR in Mashhad, Iran
Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne's disease in wild and domestic ruminants. Clinically, infected cattle show emaciation symptoms, diarrhea, and death. Asymptomatic subc...
Microbial and chemical spoilage of chicken meat during storage at isothermal and fluctuation temperature under aerobic conditions
Microbial activity and spoilage of chicken meat from the market in Mashhad, Iran, stored in air at 0, 4, 10 and 15°C was assessed. Microbial spoilage population, Total VolatileBase Nitrogen (TVB-N), pH and organoleptic...
Histomorphogenesis of pancreas in ostrich embryo (Struthio camelus)
The present study was carried out to determine the development of pancreas in ostrich embryo. For this purpose ostrich embryos obtained on 10, 13, 16, 22, 26, 30 and 36 days of prehatching and 1 day of post hatching life...
Chromosomal analysis of two buffalo breeds of Mazani and Azeri from Iran
In the present study karyotype of Mazani river buffalo was studied in comparison with those of Azeri buffalo populations from Iran. Blood samples were taken from ten (5 males and 5 females) Mazani buffaloes and thirty (1...