Therapeutic effects of granulocyte-colony stimulating factor on non-alcoholic hepatic steatosis in the rat
Journal Title: Annals of Hepatology - Year 2013, Vol 12, Issue 1
Abstract
[b]Background and rationale[/b]. Non-alcoholic hepatic steatosis refers to the accumulation of triglycerides in the liver in the absence of alcohol consumption. Granulocyte colony-stimulating factor (G-CSF) has been reported to be an effective treatment for a variety of liver diseases. We examined the possible therapeutic effects of G-CSF on non-alcoholic hepatic steatosis in rats. [b]Material and methods[/b]. Thirty-week-old Otsuka Long Evans Tokushima Fatty (OLETF) rats received water containing 30% sucrose for 8 weeks to promote the development of non-alcoholic hepatic steatosis. After development of the model, the rats were injected with G-CSF (100 μg/kg/day) or saline for 5 days. Four weeks after this treatment, serum levels of glucose, total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and free fatty acids (FFA) were measured. Histology was examined by hematoxylin and eosin (H-E) and periodic acid Schiff (PAS) staining, and levels of expression of hepatic lipogenic enzymes were determined by RT-PCR. [b]Results[/b]. The G-CSF-treated rats displayed significantly fewer lipid droplets than the saline-treated rats (P < 0.01), and their levels of sterol regulatory element-binding protein (SREBP)-1c, fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) mRNAs were also lower (P < 0.01), as were their liver weight and serum levels of TG and FFA (P < 0.05). [b]Conclusion[/b]. Our results indicate that G-CSF ameliorated non-alcoholic hepatic steatosis in the OLETF rat, and this therapeutic effect involved a reduction of SREBP-1c expression. Therefore, G-CSF deserves further study as a potential treatment for non-alcoholic hepatic steatosis.
Authors and Affiliations
Song Yi-Sun, Fang Cheng-Hu, So Byung-Im, Park Jun-Young, Jun Dae Won, Kim Kyung-Soo
Keywords
Related Articles
- EP ID EP78239
- DOI -
- Views 105
- Downloads 0
Insulin resistance and steatosis in chronic hepatitis C
In chronic hepatitis C, insulin resistance (IR) and type 2 diabetes mellitus (DM) are more prevalent than in healthy controls or in chronic hepatitis B patients. HCV infection promotes IR mainly through increased TNF-a a...
Different models in predicting the short-term prognosis of patients with hepatitis B virus-related acute-on-chronic liver failure
Background and aims. Effective assessing the prognosis of patients with end-stage liver disease is always challenging. This study aimed to investigate the accuracy of different models in predicting short-ter...
Acute Liver Failure
Acute liver failure is a rare but often catastrophic illness affecting the liver and multiple organ systems. Patients with acute liver failure require a multidisciplinary approach for adequate management. With improved...
Anatomical cardiac alterations in liver cirrhosis: An autopsy study
Background. It has been suggested that liver cirrhosis (LC), regardless of etiology, may be associated with anatomical cardiac alterations. Objective. To describe the frequency and type of macroscopical anatomic cardiac...
Celebrating fifteen years of Annals of Hepatology publication: The international, open access, clinical journal devoted to liver diseases