THERAPEUTIC L-ASPARAGINASE ACTIVITY OF BACTERIA ISOLATED FROM MARINE SEDIMENTS
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2016, Vol 8, Issue 4
Abstract
L-Asparaginase, a therapeutic enzyme used in lymphoblastic leukemia and lymphosarcoma chemotherapy which is derived mostly from the bacterial sources Escherichia coli and Erwinia sp. The long term administration of the drug leads to the development of resistant tumours and anaphylactic shock in certain individuals. Hence serologically different L-Asparaginase from novel microbial sources with enhanced therapeutic potential and immunological characteristics is an essential requirement. The marine bacteria having diverse range of potential enzymes might be a source for L-Asparaginase with novel properties, which are still unexplored. In this study, we have screened marine bacteria isolated from the coastal regions of Kerala which showed both intra and extra cellular L-Asparaginase activity. Bacillus sp. (Accession no KF142395) was found to have the highest extracellular enzyme activity (2.31 IU/ml) while Shewanella sp. (Accession no KF142390) showed maximum intracellular Asparaginase activity (2.16 IU/ml).The crude extracellular enzyme preparation from Bacillus sp. had cytotoxic effect on HL60 cell line with an IC50 value of 12.5µg/ml.
Authors and Affiliations
J. K. Arjun, B. Aneesh, T. Kavitha, K. Hari Krishnan
Keywords
Related Articles
- EP ID EP625233
- DOI 10.25004/IJPSDR.2016.080407
- Views 103
- Downloads 0
Isolation and Structure Elucidation of Isoquercitrin from the Whole Herb of Hygrophila salicifolia (Acanthaceae)
There are a large number of traditional plants, their extracts and phytoconstituents presently used in modern medicine. There is a worldwide majority of the population that still relies on plants as a source of medicine....
MICROWAVE ASSISTED SYNTHESIS AND CHARACTERIZATION OF SCHIFF BASE OF 2-AMINO BENZIMIDAZOLE
New Schiff base (2-[(1H-benzimidazol-2-ylimino) methyl]-4,6- diiodophenol) was synthesized by the condensation of aryl/hetero aromatic aldehyde (3,5diiodosalicylaldehyde) with 2- amino benzimidazole under conventional an...
STANDARDIZATION OF CHITRAKADI VATI: AN AYURVEDIC POLYHERBAL FORMULATION
Standardization of herbal formulation is essential in order to assess the quality, purity, safety and efficacy of drugs. World health organization (WHO) in 1999 has given a detail protocol for the standardization of herb...
PHARMACOGNOSTIC, PHYTOCHEMICAL ANALYSIS AND ANTI DIABETIC ACTIVITY OF DRIED LEAVES OF ABRUS PRECATORIUS – AN IN VIVO APPROACH
Diabetes mellitus is among the most common disorder in developed and developing countries, and the disease is increasing rapidly in most parts of the world. It has been estimated that up to one-third of patients with dia...
SIMULTANEOUS ESTIMATION OF DICLOFENAC SODIUM AND RABEPRAZOLE BY HIGH PERFORMANCE LIQIUD CHROMATOGRAPHIC METHOD IN COMBINED DOSAGE FORMS
A simple, accurate and reproducible HPLC method have been developed for simultaneous estimation of Diclofenac sodium and rabeprazole from their tablets formulations. A phenomenex C18 (Luna) column of length 250×7.5...