Thermodynamic characterization of the aggregation phenomena of SafranineT by spectral titration and chemometric analysis
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2014, Vol 2, Issue 6
Abstract
The dimerization constants of Safranine T have been determined by studying the dependence of its absorption spectra on the temperature in the range 30ñ70 â—¦C at different total concentrations of Safranine T (1.03×10−5, 1.44×10−5 and 1.73×10−5 M). The monomerñdimer equilibrium of Safranine T has been determined by applying MCR-ALS method on the absorption spectra. The quantitative analysis of the data of undefined mixtures, was carried out by simultaneous resolution of the overlapping spectral bands in the whole set of absorption spectra. In this work the dimerization constant of Safranine T has been determined by studying the dependence of absorption spectrum on temperature at different total concentrations of dye. The enthalpy and entropy of the dimerization reactions were determined from the dependence of the equilibrium constants on the temperature (vanÃt Hoff equation). From these results it can be inferred that the driving force of the dimerization is of enthalpic origin.
Authors and Affiliations
Masoud Karimi Goftar| Young Researchers and Elite Club, Baft Branch, Islamic Azad University, Baft, Iran, Narges Alizadeh Rayeni| Young Researchers and Elite Club, Baft Branch, Islamic Azad University, Baft, Iran, Nasrollah Moradi kor| Young Researchers and Elite Club, Kerman Branch, Islamic Azad University, Kerman, Iran
Keywords
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