Thermophilic Bacteria as a Source of Novel Polymers for Biotechnological Applications
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2016, Vol 6, Issue 4
Abstract
Aims: We analyzed thermotolerant bacterial isolates of thermophiles from various regions in South East coasts of India. Study Design: A total of forty three (9.6%) thermotolerant bacterial isolates of thermophiles were secluded out of 444 isolates obtained from water samples from the Adyar River, Marina coast, Muttukadu Backwaters, Kovalam coast (India) and a prawn hatchery. In addition a novel thermotolerant Gram positive Bacillus type bacterium from the pelletized feed of fish was examined. Results: Amongst 134 isolates that grew on E. coli FAGI agar, 23 (17%) were thermotolerant when tested at 15 psi for 30 min. Of the 72 isolates that grew on Staphylococcus Baird Parker agar 10 (13%) were thermotolerant, whilst among the 128 isolates that grew on Salmonella agar ÖNÖZ 10 (8%) were thermotolerant. In contrast, none of the 110 isolates that grew on TCBS agar (Vibrio sp.) exhibited thermotolerance, although they were isolated from the same regions as the other bacteria. The optimal temperature for the growth of most thermophiles was 45ºC, except for 9 isolates. Of the latter, 7 grew at 50ºC, one at 52ºC and one at 55ºC, respectively. There were some extremely thermotolerant bacterial isolates that exhibited consistency in profuse growth at 15 psi (121°C) for 30 min. Other isolates showed inconsistency in growth after autoclaving the cultures at 15 psi (121°C) for 30 min, and these bacteria showed development of endospores. Isolates of thermotolerant bacteria that grew on Staphylococcus Baird Parker agar and Salmonella agar ÖNÖZ were predominantly antibiotic resistant when compared with those bacterial isolates that grew on E. coli FAGI agar. A major 43 kDa toxin-protein as well as 15 other minor protein subunits was detected in the thermotolerant Bacillus sp. isolate. The proteins were toxic and caused mortality to gold fish Cyprin macrophthalmus. Conclusions: This study documents 43 thermotolerant bacterial isolates from water samples and a Bacillus-type bacteria from fish feed in Chennai region, India and these bacterial isolates offer the potential for exploitation in biotechnological applications.
Authors and Affiliations
Palaniappan Ramasamy, Aruna Sharmilli
Keywords
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- EP ID EP351810
- DOI 10.9734/JABB/2016/25675
- Views 75
- Downloads 0
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