THP-1-Derived Dendritic Cells: A Rapid and Reproducible In Vitro Model for Phagocytosis Assays Involving Tumor Cells
Journal Title: Advances in Clinical Toxicology - Year 2024, Vol 9, Issue 4
Abstract
Dendritic cells (DCs) play a vital role in antigen-specific adaptive immunity by serving as sentinels, recognizing and presenting specific pathogenic peptides. Herein, we developed a novel approach for obtaining immature DCs from THP-1 cells. We observed that THP-1-derived DCs express the classical markers CD11c and CD-209 and that their morphology is comparable with that of DCs. Furthermore, THP-1-derived DCs responded to lipopolysaccharides, as demonstrated by the high expression of the costimulatory molecules CD80, CD86, and CD83. Morphological changes were characterized by longer dendrites, and the inflammatory cytokines IL-6, TNF-α, IL-1β, MIP-1α, MIP-1β, and VEGF were released. Moreover, in THP-1-derived DCs, the transcription factors NF-ƙB, STAT1, MYD88, and MDA5 were upregulated. Lastly, these cells removed tumor cell-derived apoptotic bodies via phagocytosis. In summary, we presented a powerful, rapid, and reproducible method to generate THP-1- derived functional DCs, reinforcing THP-1 as a model for in vitro studies and an alternative strategy for using Mo-DCs.
Authors and Affiliations
Cunegundes PS , de Souza JG , Oliveira DS , de Souza MM , AlvarezFlores MP , Gomes RN , de Melo TC and Chudzinski-Tavassi AM
Keywords
Related Articles
- EP ID EP755985
- DOI 10.23880/act-16000320
- Views 33
- Downloads 0
How Bulky of Base Adducts can be Responsible for Clastogenicity rather than Mutagenicity?
Two types of TK mutants are induced by genotoxic factors; normally growing (NG) TK mutants due to point mutations of targeted TK locus, and slowly growing (SG) mutants due to gross structural changes involving the growth...
Safeguarding Public Health: The Imperative of Strong Toxic Substance Regulations
The increasing prevalence of toxic substances in our environment poses significant challenges to public health. This editorial examines the importance of robust public health policies related to toxic substance regulatio...
Herb Induced Aconite Poisoning and Bidirectional Ventricular Tachycardia
Aconite is a potent cardiotoxic compound and the cardio toxicity gets manifested by hypotension, ventricular tachycardia, and even cardiac death. Herbal medicines may contain aconite as an active gradient, and ingestion...
Pharmaceutical Applications of Circular Dichroism for Nanomaterial’s
Circular dichroism is a powerful, fast, non-destructive analytical technique for small molecules and big molecules such as proteins, peptides etc. Here, a basic review on principle and advantages of CD is given followed...
The Toxicological Studies of Securidaca longepedunculata on the Heart, Liver, Kidney and Lungs of Rats
Securidaca lonpedunculata is a savannah plant widely distributed in Africa. The plant has a reputation of broad spectrum of medicinal, pharmacological and therapeutic properties, it is reported that almost all parts of...