THREONINE MUTANT STRAIN-PRODUCER BREVIBACTERIUM FLAVUM ІМВ В-7446 CHARACTERISTICS AND OPTIMIZATION PROCESS OF ITS BIOSYNTHESIS
Journal Title: Мікробіологія і біотехнологія - Year 2015, Vol 0, Issue 2
Abstract
Aim. Identification and optimization process of biosynthesis threonine of mutant strain-producer Brevibacterium flavum ІМВ В-7446. Methods. The strain Brevibacterium flavum ІМВ В-7446 was studied using standard microbiological and biochemical methods. A fragment of genomic DNA isolated from agarose gel using a set of «Macherey-Nagel NucleoSpin Extract» according to the instructions of the manufacturer and sequenced. A comparative analysis was done by evaluation of statistical significance adjustment nucleotide sequences using the program ClustalW. Phylogenetic dendrogramm was created using a combination of techniques nearest neighbors and maximum likelihood. Results. The influence of different process parameters on the synthesis of cultivation threonine mutant strain and stability. Intensification synthesis was achieved by introducing growth factors in the culture medium. Powered dendrogramm of phylogenetic relationships investigated the strains and related the strains of Brevibacterium databases «GenBank». Conclusions. Comparative analysis of nucleotide sequences of gene 16S rRNA sequences included in the database GenBank, showed that the mutant and parent strains may belong to the genus Corynebacterium. Mutant strain had a high percentage of similarity (98%) with a species C. glutamicum. Optimization of the medium for culturing and producing the optimal cultivation parameters (temperature, pH, dissolved oxygen, the amount of inoculation, growth factors, various carbon sources) was established. Threonine strain was produced 11,9 g/dm3 by optimized cultivation conditions.
Authors and Affiliations
G. S. Andriiash, G. M. Zabolotna, A. F. Tkachenko, S. M. Shulga
Keywords
Related Articles
- EP ID EP395032
- DOI 10.18524/2307-4663.2015.2(30).48081
- Views 84
- Downloads 0
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