THYMELAEA MICROPHYLLA AMELIORATES OXIDATIVE STRESS AND METALLOTHIONEIN LEVEL INDUCED BY CADMIUM IN RAT KIDNEY
Journal Title: World Journal of Pharmaceutical Research - Year 2018, Vol 7, Issue 2
Abstract
Background and Objective: The present investigation was performed to evaluate the protective effect and the antioxidant power of Thymelaea microphylla against cadmium chloride induced oxidative stress and renal toxicity in Wistar rats. Materials and Methods: First, antioxidant and radical scavenging effect of Thymelaea microphylla was evaluated in vitro with 2,2- diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radicals assays. Then, Wistar rats were subjected to oral pretreatment with n-butanolic extract of Thymelaea microphylla (BETM) (200 mg/kg) against the renal toxicity induced by oral administration of cadmium chloride CdCl2 (5 mg/kg) for 28 days, vitamin E (250 mg/Kg) was used similarly as standard. Efficiency of Thymelaea microphylla against the renal toxicity was evaluated in terms of biochemical estimation of renal function parameters, antioxidant enzymes activities, metallothionein levels and histopathological modifications. Thymelaea microphylla antiinflammatory effect was evaluated by carrageenan (1%) induced rat paw edema. Results: Thymelaea microphylla revealed a high scavenging ability of DPPH and OH radicals. Also, Thymelaea microphylla(200 mg/Kg) pretreatment prevented deteriorative effects caused by cadmium chloride through a protective mechanism that included significant decrease in the levels of Blood Urea Nitrogen, creatinine, gamma-glutamyltranspeptidase (GGT) , potassium, sodium, Myeloperoxidase, Nitric Oxide and Thiobarbituric Acid Reactive Species as well as by restoration of antioxidant enzymes, metallothionein levels and histopathological changes against cadmium chloride administration. Additionally, Thymelaea microphylla revealed an important anti-inflammatory effect reducing carrageenan induced paw edema. Conclusion: This study proved the antioxidant and protective effect of Thymelaea microphylla against cadmium chloride induced oxidative stress and kidney injury.
Authors and Affiliations
Samira Laib
Keywords
Related Articles
- EP ID EP620013
- DOI -
- Views 121
- Downloads 0
LIPID PROFILE OF MALARIA INFESTED WISTAR ALBINO RATS TREATED WITH THE CRUDE EXTRACT OF ARTEMISIA ANNUA AND ARTEMISININ COMBINATION THERAPY.
The comparative effects of the crude extract of Artemisia annua and artemisinin combination therapy (ACT) on the lipid profile of wistar albino rats was evaluated in this study. Twenty four (24) rats used were divided in...
THE IMPORTANT ROLE OF TECHNOLOGY TRANSFER IN PHARMACEUTICAL INDUSTRY-A REVIEW
The Technology Transfer is both integral and critical to drug discovery and development process for new medicinal products this process gives necessary information for technology transfer from R&D to PDL department and d...
EFFECTS OF OXIDATIVE STRESS ON MALE REPRODUCTIVE TRACT OF MICE INFECTED WITH BRUCELLA MELITENSIS
Reproductive is important public health problem (Ruder etal., 2008). Ménézo etal.,(2012) recorded that about one out six couples were suffering from infertility in human and 40 t0 50% of the cases return to females and 3...
FACTORS ASSOCIATED WITH HYSTERECTOMY
The current study was undertaken to evaluate various factors associated with hysterectomy observed in tertiary care institute. Total 40 cases of peripartum hysterectomy were enrolled in the study Maternal characteristics...
THE PROPORTION OF PREGNANCY-ASSOCIATED ASYMPTOMATIC BACTERIURIA IN AL-KARKH HOSPITAL
The aim of the research determines the prevalence and the causative organisms of asymptomatic bacteriuria among pregnant women. A retrospective analysis was performed of the routine prenatal screening (urine culture test...