Tigecycline Resistance among Clinical Isolates of Staphylococcus aureus from North-east India
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2017, Vol 7, Issue 4
Abstract
Objective: The current study investigated the resistance against cycline group of antibiotic among clinical isolates of Staphylococcus aureus and molecular characterization with special reference to phenotypic screening of tigecycline resistance. Methods: 197 coagulase producing Staphylococcus spp. were collected from private diagnostic laboratories and Silchar Medical College and Hospital, India. Phenotypic screening was done with cycline groups of antimicrobials and genotypically characterized by PCR, targeting coa gene. Detection of tet(K), tet(M), tet(O) and tet(X) genes was performed by PCR. Antibiogram profiling was performed and clonality was also determined. Results: 127 coagulase positive S. aureus were phenotypically screened with cycline group of antibiotics in which prominent resistant type showed highest non-susceptibility against tigecycline 53.5% (n=68) while lowest against minocycline 20.5% (n=26). Multiplex PCR assay showed that tet(K) gene to be the most common cycline group of antimicrobial resistance determinant in S. aureus, followed by tet(M) gene. Multiple genes were also observed. However, resistant gene tet(O) was not reported. Antibiotic susceptibility testing and MIC for therapeutic purpose showed highest susceptibility rate against linezolid. DNA fingerprinting experimented with Pulsed Field Gel Electrophoresis had shown seven pulsotypes. Conclusion: Resistance to cycline group of antibiotics with special reference to tigecycline is not frequently reported from India. Thus, this study is of clinical interest in terms of selection of proper antimicrobial chemotherapy and also using tigecycline for phenotypic screening of cycline group of antibiotic resistance in Staphylococcus aureus. J Microbiol Infect Dis 2017; 7(4):173-177
Authors and Affiliations
Swati Sharma, Deepshikha Bhowmik, Shiela Chetri, Deepjyoti Paul, Debadatta Dhar Chanda, Atanu Chakravarty, Amitabha Bhattacharjee
Keywords
Related Articles
- EP ID EP413783
- DOI 10.5799/jmid.368660
- Views 86
- Downloads 0
Streptokok Faranjitinin Süpüratif Olmayan Komplikasyonlarını İrdeleyen Prospektif Bir Çalışma
Amaç: Grup A Streptokoklar (GAS) faranjitin en önemli bakteriyel nedenidir, çünkü antibiyotiklerle tedavi edilebilir ve tedavi başarısızlığı süpüratif ve nonsüpüratif komplikasyonlara neden olabilir. Nonsüpüra...
Middle East Respiratory Syndrome: MERS
Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerging respiratory virus. It was first identified in Saudi Arabia in 2012. MERS-CoV infection is characterized by a spectrum of illness ranging from mi...
Pleuropulmonary Parasitic Infections of Present Times-A Brief Review
Pleuropulmonary Parasitic Infections of Present Times-A Brief Review Isabella Princess1, Rohit Vadala2 1Department of Microbiology, Apollo Speciality Hospitals, Vanagaram, Chennai, India 2Department of Pulmonary and Crit...
Servikal vertebra ve akciğer tutulumu ile seyreden bruselloz: Nadir bir olgu
Brusellozun yaygın görülen bir komplikasyonu olan kas-iskelet sisteminde vertebra tutulumu sıktır. Literatürde servikal spondilodiskit ve pulmoner tutulumun birlikte olduğu vaka tespit edilmemiştir.Bu çalışmada bir aydı...
Eruptive Xanthoma during Antiretroviral Therapy
We present a case of eruptive xanthoma in a man living with HIV after changing antiretroviral therapy. People living with HIV are at increased risk of dyslipidemia due to the infection itself and the use of antiretrovira...