TIGIT+ immunoregulatory T cells upregulate autophagy-related proteins
Journal Title: Tumor Microenvironment Research - Year 2020, Vol 2, Issue 3
Abstract
Background: CD4+CD25+ Foxp3+ regulatory T cells are essential for immune homeostasis. Naive Treg cells can undergo further differentiation into an effector state in response to antigen and a variety of environment cues. However, the potent mechanisms are poorly understood. Autophagy maybe one of the potent mechanisms participate in the differentiation process. Methods: The gene expression profiles of GSE72494 were downloaded from Gene Expression Omnibus database and differently expressed genes were filtered by EdgeR package and R. TIGIT+ Treg and TIGIT− Treg cells were separated by magnetic beads and flow cytometry. RT-PCR was used to determine the mRNA level and the protein expression of autophagy-related proteins was determined by western blot assay. Results: In total, 1,896 DEGs were identified between TIGIT+ and TIGIT− Treg cells, including 1,280 up-regulated genes and 616 down-regulated genes. Three autophagy related genes，MAP1LC3A (LC3), SQSTM1 (P62) and BECN1 (Beclin1) were increased in TIGIT+ Treg cells. Mechanistic target of rapamycin, a key molecule may have the function of inhibiting autophagy was down-regulated in TIGIT+ Treg cells. Autophagy-related proteins LC3, P62 and Beclin1 were significantly increased and the expression of mTOR was significantly decreased in TIGIT+ Treg cells. Conclusion: TIGIT+ Treg cell is a subset of Treg cells with unique functions. Autophagy-related processes may be involved in the differentiation of TIGIT− Treg into TIGIT+ Treg cells. Inducing the generation of TIGIT+ effect Tregs may be a new way to prevent the immune rejection of acute organ transplantation
Authors and Affiliations
Bo-Zhao Ma
Keywords
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- EP ID EP685214
- DOI 10.12032/TMER202007250703
- Views 202
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