Time-dependent changes of immunologic responses after burn injury and immunomodulation by cimetidine and pyrimethamine in an animal model
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2010, Vol 23, Issue 4
Abstract
Severe suppression of the immune system is the major cause of infections following burn injury. The aim of this study was to investigate the time-related alterations of immune responses following thermal injury in an animal model and also to modulate immune responses by use of the immunomdulators cimetidine and pyrimethamine. Male Balb/c mice were anesthetized and given a 10% total body surface area full-thickness burn. The time-dependent changes of delayed type hypersensitivity (DTH) and antibody responses to sheep red blood cell (SRBC) were assessed at post-burn days (PBD). The effects of different doses of cimetidine and pyrimethamine on DTH response were also quantitated at 10 PBD. Marked suppression of DTH response occurred during 30 days after burn trauma, with maximal suppression occurring between 10 to 14 days after burn injury. Simultaneously the antibody response to SRBC was significantly increased after thermal trauma. Cimetidine (at doses of 10 and 15 mg/kg) and pyrimethamine (at doses of 5 and 10 mg/kg) significantly augmented DTH response after thermal injury. These results showed that the severe time-dependent alterations occurred in DTH and antibody responses following burn injury. Cimetidine and pyrimethamine also restore burn-induced suppression of DTH response following thermal trauma.
Authors and Affiliations
Jafarzadeh A, Hassan ZM, Ebtekar M, Mohaghegh-Hazrati S, Tiraihi T
Keywords
Related Articles
- EP ID EP155151
- DOI -
- Views 84
- Downloads 0
Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract
The flowers of African marigold (Tagetes erecta L), a medicinal plant widely cultivated in Thailand, were subjected to evaluation for total phenolics, DPPH scavenging and thiobarbituric acid-reactive substance (TBA...
Effect of repeated oral therapeutic doses of methylphenidate on food intake and growth rate in rats
Central nervous system stimulants are known to produce anorexia. Previous data suggest that methylphenidate can have variable effects on caloric intake and growth rate. A dose-response study was performed to monito...
Rapid and simple DNA extraction protocol from goat rumen digesta for metagenomic analysis
In contrast to the traditional culturing techniques and microscopy that have led to the identification and characterization of only about 15-20 % of the rumen microbes till date, nucleic acid-based molecular approa...
Formulation and evaluation of suspensions: Mefenamic acid prodrugs
Gastrosparing novel prodrugs (MAM and MAT) synthesized consisted of mefenamic acid (MA) with menthol (M) and thymol (T). Structural characterizations of synthesized esters were done by Infra red spectroscopy (IR),...
Synthesis and evaluation of mannich bases of benzimidazo [1,2-c] quinazolin- 6(5h)-thione for antimicrobial activity
The intermediate Benzimidazo[1,2-c] quinazolin-6(5H)-thione (1) was obtained by cyclization of 2-(2’-aminophenyl) benzimidazole with carbon disulfide. Mannich base (2a-d) of compound (1) was obtained on treatment with Pa...