Tissue culture and rapid propagation of Dendrobium cariniferum

Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 9

Abstract

"In order to establish a rapid propagation system for Dendrobium cariniferum Rchb. f., the immature seeds were selected as explants to study the suitable medium for for tissue culture, and mediums aseptic seed germination. Meanwhile, the appropriate multiplication conditions of protocorm and buds were selected subculture of protocom, and the rooting medium and transplanting matrix were also screened. The results showed that the medium of MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+ sucrose 20 g/L had a significant effect on seedlings germination and protocorm induction of D. cariniferum. The medium of MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+sucrose 20 g/L+activated carbon 1.0 g/L was suitable for protocorm proliferation and shoot induction, and the propagation coefficient reached 5.8 times after cultured 90 d. While the optimal medium for rooting was MS+6-BA 1.0 mg/L+KT 0.5 mg/L+NAA 0.2 mg/L+sucrose 30 g/L+banana mud 100 g/L+activated carbon 1.0 g/L, the rooting rate reached 100% and the roots had higher quality and quantity after cultured 90 d. The optimum time for the transplantation of root regeneration plants was March and April in Guilin area, and sphagna was suitable as media for transplanting and domestication, and the rooted plants had a survival rate of 76.7%."

Authors and Affiliations

Shuo QIU, Feng-luan TANG

Keywords

Related Articles

Introduction, cultivation and nutritional analysis of‘Zhongtian’rose in Huaiji, Guangdong

‘Zhongtian’rose was introduced in Huaiji, Guangdong, and the agronomic traits were compared among the 1-year , 2-year and 3-year seedlings . The contents of amino acids, mineral elements, polysaccharides, total phenols a...

Effects of different method of loading tobacco leaves on aroma content of tobacco leaves

"In order to study the effects of different tobacco loading methods on neutral aroma components of tobacco leaves,we conducted a comparative experiment between tobacco clamp,loose leaves accumulation and sticking and i...

Comparative analysis of carbon footprint of agricultural products supply chain based on life cycle

Currently, researches about rapid and healthy development of agricultural products circulation had drawn a great attention with the sustained growth of economy and upgrade of family consumption. Using the life cycle esti...

Separating,screening and identification of an antimicrobial endophytic bacterium from Ficus microphylla L.f.

"This study was done to screen and identify the endophytes with antibacterial activity from Ficus microcarpa L.f. in order to develop biocontrol agents. Endophytes were isolated by tissue block method and tissue homogena...

Identification and antifungal activity against plant pathogens of actinomycetes strain A50

"This study aimed to obtain actomycetes with high activity against plant pathogens from soil. Soil samples were collected from woodlands of Lake Chi’ao in Shenzhen. Actinomycetes strain A50 was isolated using serial dilu...

Download PDF file
  • EP ID EP601578
  • DOI 10.16768/j.issn.1004-874X.2018.09.008
  • Views 59
  • Downloads 0

How To Cite

Shuo QIU, Feng-luan TANG (2018). Tissue culture and rapid propagation of Dendrobium cariniferum. 广东农业科学, 45(9), 48-52. https://europub.co.uk/articles/-A-601578