Tissue culture and rapid propagation of Dendrobium cariniferum
Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 9
Abstract
"In order to establish a rapid propagation system for Dendrobium cariniferum Rchb. f., the immature seeds were selected as explants to study the suitable medium for for tissue culture, and mediums aseptic seed germination. Meanwhile, the appropriate multiplication conditions of protocorm and buds were selected subculture of protocom, and the rooting medium and transplanting matrix were also screened. The results showed that the medium of MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+ sucrose 20 g/L had a significant effect on seedlings germination and protocorm induction of D. cariniferum. The medium of MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+sucrose 20 g/L+activated carbon 1.0 g/L was suitable for protocorm proliferation and shoot induction, and the propagation coefficient reached 5.8 times after cultured 90 d. While the optimal medium for rooting was MS+6-BA 1.0 mg/L+KT 0.5 mg/L+NAA 0.2 mg/L+sucrose 30 g/L+banana mud 100 g/L+activated carbon 1.0 g/L, the rooting rate reached 100% and the roots had higher quality and quantity after cultured 90 d. The optimum time for the transplantation of root regeneration plants was March and April in Guilin area, and sphagna was suitable as media for transplanting and domestication, and the rooted plants had a survival rate of 76.7%."
Authors and Affiliations
Shuo QIU, Feng-luan TANG
Keywords
Related Articles
- EP ID EP601578
- DOI 10.16768/j.issn.1004-874X.2018.09.008
- Views 78
- Downloads 0
Investigation and evaluation on soil fertility of cassava plantations in Guangdong
"Samples of the top soil (0 to 20 cm beneath the ground) from cassava plantations in five demonstration counties (Xingning, Wengyuan, Suixi, Kaiping, and Yunan) of CARS-12 Guangzhou Comprehensive Test Stationb , were sel...
Effect of cadmium on the physiological changes of Clerodendrum bungei Steud.
"In order to determine the effect of cadmium on the physiological changes of Clerodendrum bungei seedlings，field experiment was conducted with 5 Cd concentrations as 0，0.1，0.3，0.6，0.9 mmol/L. Based on chlorophyll fl...
Identification of an endophytic bacteria from Pennisetum sp. and the preliminary analysis of its growth promoting properties
"Using Juncao as materials，endophytes were gained by tissue isolation and streak plate method. The ability of indole acetic acid（ IAA） production and siderophore production were tested by the Salkowski colorimetric me...
Effects of different nitrogen concentrations on growth and chemical composition of a freshwater microalga Scenedesmus obliqnus
An experiment was carried out to evaluate the effects of different nitrogen concentrations on growth and chemical composition of a freshwater microalga Scenedesmus obliqnus. In this study，the culture solutions were desi...
Research advances on breeding of pharmaceutical Dendrobium in China
Dendrobium is a kind of traditional and precious Chinese medicinal material, which has the functions of nourishing stomach, nourishing yin and clearing heat, moistening lung and relieving cough, strengthening body, enhan...