To Assess the Time Since Death on Morphological Changes of Neutrophils, Lymphocyte, Monocytes & Eosinophils: An Autopsy Based Study
Journal Title: International Journal of Medical Research Professionals - Year 2018, Vol 4, Issue 4
Abstract
Background: In Medico-legal work the determination of the accurate time passed since Death has always been important. Therefore, this study was undertaken to study the estimation of time since death by morphological changes in neutrophils, lymphocytes & monocytes. Materials & Methods: The hospital based observational study was carried out on 150 cases in Department of Forensic Medicine, RNT Medical College, Udaipur. All the corpses were kept in deep freezer at 40 C after certified death in the attached hospital. The morphological changes observed in neutrophils, lymphocytes, Monocytes & eosinophils were observed in terms of change in their appearance, shape, central pallor, integrity and lytic activity in the cells and their internal structures. Results: In this study, males were more preponderant as compare to female, male to female ratio was 4:1. Neutrophils were found to be recognizable latest by 30 hours & Lymphocytes were found recognizable latest by 24 hours after death in present study. There was no disintegration observed in monocytes during the 1st six hours after death in any case. Conclusion: The present study proposes that lymphocytes are the most resistant blood cells as regards to degeneration after death. Although a single cell change viewed in isolation may not do wonders in framing opinion of time elapsed since death but a study of morphological appearance of various cells at the time of examination may lead to meaningful inferences.
Authors and Affiliations
Anis Ahmed
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