To evaluate the combinational effect of Ethyl Pyruvate, Berberine and Poloxamer 188 on 3 nitropropionic acid induced Huntingtons disease
Journal Title: Innovations in Pharmaceuticals and Pharmacotherapy (IPP) - Year 2018, Vol 6, Issue 2
Abstract
The present study is undertaken to study the combinational effect of ethyl pyruvate, berberine, and poloxamer 188 on 3-nitropropionic acid (3NP)-induced Huntington’s disease. 3NP is a well-versed experimental model to study Huntington’s disease (HD)-induced motor, memory, and mitochondrial dysfunctions. Ethyl pyruvate, berberine, and poloxamer 188 are reported to exhibit a neuroprotective effect in various animal models. Moreover, berberine is reported to have a protective effect on memory dysfunction. In addition, poloxamer-188 and ethyl pyruvate reduce muscular atrophy, neuronal loss, and neuroinflammation. These evidences suggest that either single or combination of these agents may protect against 3NP model. Administration of 3NP (10 mg/kg) for 14 days in male Wistar rats significantly induced HD-like symptoms in rats as indicated by reduced locomotor activity, body weight, grip strength, oxidative defense, cognition, and mitochondrial complex enzymes activities in striatum, cortex, and hippocampus. All the behavioral and biochemical studies on control and treatment groups were done after 14 days. Marked elevation in lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), and lipid peroxidation was also observed. Furthermore, a decrease in the levels of reduced glutathione was noted. However, pretreatment of combination (ethyl pyruvate [40 mg/kg], berberine [50 mg/kg], and poloxamer 188 [40 mg/kg]) significantly attenuated behavioral alterations, oxidative stress, neuronal loss, and mitochondrial enzymes complex dysfunction in 3NP-treated group and potentiates their respective protective effects.In conclusion, a combination of ethyl pyruvate, poloxamer 188, and berberine could be used to manage behavioral and biochemical alterations in HD than either of single drug therapy
Authors and Affiliations
Pallavi Bhosle
Keywords
Related Articles
- EP ID EP570163
- DOI -
- Views 76
- Downloads 0
Advances and shortcoming of HIV/AIDS therapy
Increasing incidence of resistance of anti-tubercular drugs worldwide and the beginning of multi drug resistant in tuberculosis raise the need of new drugs for the treatment of tuberculosis and to understand the advances...
Formulation and evaluation of novel herbal antidiabetic transdermal patch
Aim: The aim of the present study was to formulate and evaluate the Transdermal Drug Delivery System of an antidiabetic herbal drug. And to increase the efficacy and to improve the patience compliance of the herbal medic...
Evaluation of cardioprotective activity of Woodfordia fruticosa flower extracts against doxorubicin induced cardiotoxicity
Woodfordia fruticosa (WF) flower extracts (Petroleum ether- PEWF; Chloroform - CHWF; Ethyl acetate- EAWF; Methanol- MEWF; Hydroalcohol - HAWF) was evaluated for the phytochemical contents using phytochemical screening an...
Virulence factors and antibiotic sensitivity pattern in E. coli isolated from extra intestinal infections in a tertiary care hospital
Identification of virulence determinants among the clinically isolated microorganisms assumes a greater significance in the patient management perspective. Aim: To detect the virulence factors produced by Escherichia. co...
Phophodiesterase-4 enzyme inhibitors as potential anti-inflammatory agents
The Phosphodiesterases (PDEs) are a diverse family of enzymes that hydrolyze cyclic nucleotides such as cAMP & cGMP to the inactive 5’-AMP and 5’-GMP, respectively and thus play a key role in regulating intracellular lev...