To Identify the Prevalence Leptospira Serogroups in the Cases From Southern Gujarat Region
Journal Title: National Journal of Laboratory Medicine - Year 2016, Vol 5, Issue 2
Abstract
Introduction: Leptospirosis, is a widespread zoonosis which affects the human worldwide. It is an acute febrile illness; the severity varies from mild to severely fatal form. Because of protean clinical manifestation and unavailability of appropriate laboratory diagnostic facilities in most part of the country, human cases has been reported less in India. Clinically leptospirosis shows, similar signs and symptoms with other diseases which mandate the need of laboratory confirmation. Aim: To identify prevalent circulating serogroups in human cases of South Gujarat region by performing Microscopic Agglutination Test (MAT) and to compare results of commercially available IgM Enzyme-Linked Immunosorbent Assay (ELISA) with MAT. Settings and Design: A retrospective cross sectional study. Materials and Methods: A total of 157 serum samples were collected from suspected leptospirosis patients and subjected to serological tests like MAT and IgM-ELISA. Identification of predominant infecting serogroup was done by using panel of 11 serovars. Statistical Analysis Used: Sensitivity and specificity. Results: MAT analysis of 157 samples showed 113(72%) to be positive. Antibodies were predominantly against serogroups like Leptospira autumnalis (60%), L. australis (16%) and L. pyrogenes (7%). IgM-ELISA showed a positivity of 74% compared to MAT. Conclusion: L. Autumnalis is the predominant serovar identified in the present study so MAT can be used as a gold standard in conjunction with an IgM-ELISA in diagnostic laboratories.
Authors and Affiliations
Dr. Tanvi Panwala, Dr. Summaiya Mulla
Keywords
Related Articles
- EP ID EP378974
- DOI 10.7860/NJLM/2016/17772:2109
- Views 144
- Downloads 0
Extended-Spectrum β-lactamase Production among Enterobacter cloacae and Enterobacter aerogenes at a Tertiary Care Center in Coastal Karnataka
Introduction: Enterobacter spp. have been increasingly isolated as nosocomial pathogens. Multi-drug resistance is more frequently noted among these pathogens. Extended spectrum beta lactamases (ESBLs) are difficult to de...
Mucoepidermoid Carcinoma of the Head and Neck: Clinico-Pathologic Study of 12 Cases
Introduction: Mucoepidermoid carcinoma (MEC), one of the most common salivary gland malignancies, but it is rare in children. MEC occurs mainly in the parotid gland, followed by the minor salivary glands. Palate being th...
Diagnostic Conundrum in a Case of Solid Papillary Renal Cell Carcinoma
Papillary Renal Cell Carcinoma (RCC) is the second most common carcinoma arising from the renal tubular epithelium. It comprises 10-15% of cases in surgical series. The solid variant of papillary RCC includes only those...
Distribution of ABO, Rhesus Blood Groups, Transfusion Transmitted Diseases and Adverse Donor Reactions in a Blood Bank of a Tertiary Care Hospital: A Five Year Study
Introduction: Study of the distribution of A, B, O blood group systems is essential for genetic studies, medico – legal issues and importantly for efficient delivery of blood bank services. Also important is the immunolo...
Non Specific Inflammation: A Waste-Basket Diagnosis Harboring Cysticercus–Report of Two Cases
Cysticercosis is a well known parasitic infestation occurring at multiple sites including brain, muscles, liver, lungs, heart and peritoneum. Clinical presentation may be misleading most of the times. We herewith report...