To Study the Effect of pH on Lipase
Journal Title: International Journal of Research and Review - Year 2018, Vol 5, Issue 8
Abstract
Bacteriallipases are commercially more important mainly because of the ease of their cultivation and optimization to obtain higher yield. The industrial demand of new sources of lipases with different catalytic characteristics stimulated the isolation and selection of Bacillus subtilis. This study was designed to investigate physicochemical parameters for optimized lipase production. Microbial lipases catalyze both the hydrolysis and synthesis of long-chain acylglycerols. They are currently given much attention with the rapid development of enzyme technology. The characterization of these enzymes of paramount importance to establish the process conditions for subsequent application. To determine the optimum pH, we used the sodium citrate buffer pH 3, 3.6and 5.6 and sodium phosphate buffer at pH 7.3 and 8.Change in pH also alters an enzyme’s shape. Different enzymes work best at different pH values. The enzyme can continue locking with a substrate and continue its catalysts activity. If the pH is too high or too low it can change the shape of the enzyme. The most favourable pH value the point where the enzyme is most active is known as the optimum pH. Extremely high or low pH values generally result in complete loss of activity for most enzymes pH is also a factor in the stability of enzymes. As with activity for each enzyme, there is also a region of pH optimal stability.
Authors and Affiliations
Praveena Gupta
Keywords
Related Articles
- EP ID EP482142
- DOI -
- Views 57
- Downloads 0
The Rationality Assumption from Birth to Reincarnation
Being a very age old yet a dynamically modern social science, economics has journeyed a lot all throughout centuries. The birth dates back to Greeks before Socrates. All this while up until now many notions have seen a p...
Adverbs in Karonese
This article describes about the use and functions of adverbs in Karonese language. Adverbs in Karonese language describes the meaning of the whole sentence and describe the meaning of the sentence elements in a sentence...
Factors Affecting the B.Sc. Nursing Education among Nurses at Teaching Hospital, Jaffna, Srilanka
Bachelor of Science in Nursing (B Sc. N) education is increasingly necessary for nurses to keep abreast of rapid changes in patient care due to advancement in knowledge and technology. But many factors affect the Nurses’...
Antimicrobial Utilization Study in Indoor Patients of Paediatric Ward of a Tertiary Care Teaching Hospital
Introduction: Antimicrobial prescribing pattern analysis is very important for judicial use of antimicrobials. Antimicrobials are commonly prescribed in paediatric population. Irrational antimicrobial prescriptions along...
Development of Mathematics Learning Devices Oriented on Problem-Based Learning Model to Improve Critical Thinking Ability of Students in Across of Sma Kuala Nagan Raya Aceh
This study aims to produce mathematics learning devices which are valid, practical and effective through the Problem-Based Learning model to improve critical thinking in across of high school students in Kuala Nagan Raya...