Total phenols and flavonoids and antioxidant activity of Lampaya medicinalis F. Phil. leaf extracts
Journal Title: Journal of Pharmacy & Pharmacognosy Research - Year 2013, Vol 1, Issue 1
Abstract
Context: Lampaya medicinalis F. Phil. (Verbenaceae) is a plant that grows in northern of Chile and it is used in ethnomedicine to treat bone pain, as an analgesic, in kidney ailments, disorders of the prostate, liver disease and dyspepsia, although there are few scientific studies about it. Aims: To determine the antioxidant activity of different extracts from leaves of L. medicinalis. Methods: Extractive methods were performed in water and methanol maceration, decoction and infusion from leaves of L. medicinalis. Phenols and flavonoids were measured and evaluated the total antioxidant capacity in vitro by discoloration radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and inhibition of the enzyme xanthine oxidase (XO). Results: The L. medicinalis extract, obtained by maceration in methanol (LM-M), showed higher concentrations of phenols (5807.9 ± 340.0 mg of gallic acid equivalents/100 g dry sample), while the decoction (LM-Dx) showed higher concentration flavonoids (136.0 ± 5.5 mg of quercetin equivalents/100 g dry sample) with statistically significant differences (p <0.05) relative to the other extracts. Moreover, the best results for the DPPH method were to the extracts LM-M and LM-Dx (IC50 = 24.9 ± 3.6 and 24.1 ± 2.7 μg/mL, respectively), without significant differences between them (p> 0,05). However, extracts had no activity on XO. Conclusions: Polar extracts from leaves of L. medicinalis show antioxidant activity due to the presence of flavonoids and phenols that could justify the ethnomedical use of the plant in pathologies transiting by a component of oxidative stress.
Negative inotropic and dromotropic actions of SiO2 nanoparticles on isolated rat hearts: Effects on Na+ and Ca2+ currents
Context: SiO2 nanoparticles (NP) are widely used in the industry and in varied biotechnological and medical applications. However, epidemiological studies suggest that pollution with fine particles (in which silica is an...
Selective cytotoxic effect of 1-O-undecylglycerol in human melanoma cells
Context: 1-O-alkylglycerols are ether-linked glycerols derived from shark liver oil and found in small amounts in human milk. Previous studies showed antineoplastic activity for this family of compounds, structurally rel...
Microencapsulation by spray drying of Lannea microcarpa extract: Technological characteristics and antioxidant activity
Context: A functional extract from Lannea microcarpa (Lm), possess interesting antioxidant and anti-inflammatory properties. However, the unprocessed dried extract occurs as sticky and low-water-soluble material showing...
Pharmaceutical intervention in menopausal patients with hormone replacement therapy in a community pharmacy from Antofagasta
Context: Hormone replacement therapy (HRT) is the most widely used treatment for controlling the effects of menopause. This type of therapy causes some drug-related problems (DRP), which requires monitoring to control th...
In vitro and in vivo modulation of LPS and carrageenan-induced expression of inflammatory genes by amitriptyline
Context: Amitriptyline, a tricyclic antidepressant is used for the management of psychological disorders and various types of pain. In the previous work, it is founded that amitriptyline inhibited the migration of polymo...