TRENDS OF MICROBIOLOGICAL PROCESSES IN PRESERVED CORN ROLLED GRAIN UNDER THE INFLUENCE OF BACILLUS SUBTILIS
Journal Title: Сільськогосподарська мікробіологія - Year 2018, Vol 28, Issue
Abstract
Objective. To study the trends of microbiological processes, changes in the level of acidity and the ratio of accumulated organic acids in preserved corn rolled grain due to the influence of selected strains of Bacillus subtilis, promising for the creation of biological preservatives on their basis. Methods. Microbiological, zootechnical. Results. Grain processing with suspensions of the test strains of B. subtilis promotes the intensive development of epiphytic lactic acid bacteria in feed at day 15 of preservation, with subsequent gradual decrease at day 30 and 70 of the experiment. The number of lactic acid bacteria at day 14 after opening samples of preserved corn rolled grain is a tenfold higher than in the control variant (without the additional application of microorganisms). The dynamics of yeast growth in the feed due to the effect of the test strains of B. subtilis is characterized by their intensive development at day 15 with a gradual decrease in the number in all variants at day 30 and 70 of preservation. The number of moulds in the control variant exceeds the maximum allowable level (up to 5×104 CFU per 1 g feed) and is 2×105 at day 7 and 6.2×105 at day 14 after opening and access of air to the thickness of the preserved mass. In experimental variants, the number of moulds is smaller compared with the control both in the process of preservation and after opening the feed. The optimum pH and the ratio of organic acids at day 14 after opening has been registered in the rolled grain treated with B. subtilis B1, B. subtilis B6y and B. subtilis 44-p. Conclusion. The use of selected B. subtilis strains to preserve moist corn rolled grain promotes the establishment of the optimum level of acidity of the feed and the ratio of organic acids, inhibition of the development of undesirable microorganisms.
Authors and Affiliations
M Perederii, N Kravchenko, L Bozhok, O Dmytruk
Keywords
Related Articles
- EP ID EP498152
- DOI -
- Views 81
- Downloads 0
MORPHOLOGICAL AND CULTURAL, PHYSIOLOGICAL AND BIOCHEMICAL PECULIARITIES OF MICROMYCETES STRAINS INCLUDING IN THE COMPOSITION OF TRICHODERMA HARZIANUM 128 ASSOCIATION
Objective. Screen the active cellulolytic strains of Trichoderma micromycetes, investigate their morphological and cultural, physiological and biochemical properties for further use in the composting of organic substrate...
PRODUCTIVITY OF POTATOES MICROPLANTS IN VIVO GROWN UNDER THE ACTION OF DIFFERENT LIGHTNING SPECTRA
It has been established that the use of additional lightning by phyto LEDs under cultivation of potatoes microplants provides a positive effect on the survival and productivity of potatoes microplants in open ground cond...
ECOLOGICAL AND MICROBIOLOGICAL ASSESSMENT OF SEWAGE SLUDGE OF WASTE TREATMENT PLANTS IN THE CITY OF ODESA
Objective. Investigate the number of microorganisms – representatives of various ecological trophic groups in mechanically dewatered and naturally disinfected sewage sludge (SS) of the biological sewage treatment plants...
INFLUENCE OF BIOPREPARATION RHYZOHUMIN AND PLANT GROWTH REGULATOR BIOGLOBINE ON SYMBIOTIC NITROGEN-FIXING SYSTEM AND YIELD OF SOYBEAN
The paper presents the results of study on the influence of pre-sowing treatment of soybean seeds with the microbial preparation Rhyzohumin and growth regulator Bioglobine, and their separate application (Rhyzohumin was...
BACTERIAL TRANSLOCATION OF PROBIOTIC LACTOBACILLI
The paper presents the results of studying of the dynamics and characteristics of the translocation of two probiotic strains of lactic acid bacteria in the blood and parenchymal organs of animals. The dependence of the b...