Trombinol, a bioactive fraction of Psidium guajava, stimulates thrombopoietin expression in HepG2 cells
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 5
Abstract
Objective: To study the regulation of trombinol on thrombopoietin, an essential regulator of thrombocyte production. Methods: Effect of trombinol on thrombopoietin regulation was evaluated at the mRNA and protein levels in human hepatoma HepG2 cells. The mRNA expressions were revealed by PCR and real-time PCR, while the protein expressions were analyzed using western blotting and human ELISA kit. Statistical differences between the test were determined by student's t-test with P < 0.05 was considered statistically significant. Results: Trombinol significantly increased the expression of thrombopoietin at the level of mRNA and protein secretion in HepG2 cell lines. Trombinol with the concentration of 15 mg/mL, positively induces 2.5-fold of thrombopoietin expression. Up-regulation of GABP, a transcription factor of thrombopoietin, is suggested to be involved in cellular regulatory mechanisms of trombinol. Here, our result shows convincing evidence that trombinol affects the thrombopoietin productions in vitro. This molecular explanation of thrombopoietin's stimulating function is in line with the traditional use of Psidium guajava for treatment of diseases involving thrombocytopenia. Conclusions: Thrombopoietin stimulating function of trombinol could be potentially considered as one of alternative treatment for thrombocytopenia-related cases, including post chemotherapy shock, dengue fever and liver failure.
Authors and Affiliations
Guntur Berlian, Olivia Mayasari Tandrasasmita, Raymond Rubianto Tjandrawinata
Keywords
Related Articles
- EP ID EP253737
- DOI 10.1016/j.apjtb.2016.09.010
- Views 68
- Downloads 0
Direct detection of rpoB and katG gene mutations of Mycobacterium tuberculosis in clinical samples
Objectives: To study the rpoB and katG gene mutation rate and its markers. Methods: Cross-sectional study methods were used to study Tuberculosis. A total of 45 sputum samples were collected from Annapurna Neurological I...
Phytochemical analyses, antimicrobial and antioxidant activities of stem bark extracts of Distemonanthus benthamianus H. Baill. and fruit extracts of Solanum torvum Sw. from Gabon
Objective: To evaluate the phytochemical constituents, antimicrobial and antioxidant activities of the extracts of Distemonanthus benthamianus (D. benthamianus) stem bark and Solanum torvum (S. torvum) fruit which have b...
Development of new lateral-flow immunochromatographic strip using colloidal gold and mesoporous silica nanoparticles for rapid diagnosis of active schistosomiasis
Objective: To develop a new sandwich based lateral flow immunochromatographic strip for rapid detection of circulating Schistosoma mansoni antigen in serum and urine samples of patients with active schistosomiasis. Metho...
Antioxidant and antiglycation properties of two mango (Mangifera indica L.) cultivars from Senegal
Objective: To evaluate the total phenolic contents, antioxidant and antiglycation activities of leaves, barks, roots and kernels from two cultivars of Mangifera indica (Anacardiaceae). Methods: Total phenolic contents we...
The potential of standardized quassinoid-rich extract of Eurycoma longifolia in the regulation of the oestrous cycle of rats
Objective: To evaluate the effects of Eurycoma longifolia (E. longifolia) standardized extract on the oestrous cycle, levels of reproductive hormones and histology of the ovaries of Sprague-Dawley rats. Methods: Female r...