Tumor budding as a new histological parameter in the metastasis of colorectal cancer
Journal Title: Progress in Health Sciences - Year 2018, Vol 8, Issue 2
Abstract
Introduction:The presence of tumor budding, i.e.,single cancer cells or a nest of poorly differentiated cells at the front of tumor invasion appears to be a new histopathological indicator of increased aggressiveness of colorectal carcinoma. Purpose: The aim of this work was a retrospective evaluation of the invasion front (tumorbudding, vascular invasion,and lymphocytic infiltration) in postoperative biopsies of patients with colorectal carcinoma and analysis of the 5-year survival. Materialsand methods:The study was based on the material received after surgical treatment of 164 patients with colon cancer. Tissue was obtained directly following tumor resection, fixed in 10% formaldehyde and embedded in paraffin blocks using a routine method by melting with paraffin at a temperature of 56º C. These samples were then routinely stained with haematoxylin and eosin and underwent a histopathological evaluation, with particular attention being paid to the invasion front of the tumor. The immunohistochemical expression of cytokeratin 20 was also evaluated using anti-human CK20 monoclonal antibody (clone Ks.20.8, Dako, Poland). Results: Tumor budding was found in 124 out of 164 patients. Statistical analysis showed a correlation between the presence of tumor budding TB and depth of invasion (pT), lymph node metastasis, distant metastasis, lymphocytic infiltration,and vascular invasion. The cumulative five-year survival correlated with the lack of tumor budding and vascular invasion, as well as a decrease in lymphocyticinfiltration.<br/><br/>
Authors and Affiliations
W. Ustymowicz
Keywords
Related Articles
- EP ID EP431345
- DOI 10.5604/01.3001.0012.8332
- Views 59
- Downloads 0
DIPNECH – a preneoplastic lesion or a separate biological entity?
Introduction: DIPNECH is a very rare condition with under 100 cases reported in the scientific literature worldwide. The new WHO classification of lung tumors considers it as a preneoplastic condition, leading to further...
Evaluation of threat of mobile phone – addition among Belarusian University students
Introduction: The use of mobile phones has increased worldwide during the last decade, especially in adolescents. Objective: The assessment role of mobile phone in the students' life, evaluation of the mobile phone addit...
Significance of mucin expression in pancreatic intraepithelial neoplasia (PanIN) – precursor lesions of pancreatic ductal adenocarcinoma (PDAC)
Purpose: To evaluate chosen mucins (mucin 1, 4, 5AC) expression in pancreatic intraepithelial neoplasia, which is a precursor lesion of pancreatic ductal adenocarcinoma. Materials and methods: The study group included 70...
Blood flow parameters in temporal short posterior ciliary arteries in myopic patients
Introduction: Myopia is the most common refractive defect. Enlargement of the defect is the result of lengthening of the eyeball. This leads to the development of degenerative changes in the retina and choroid of the eye...
Healthy behaviors in Polish children and adolescents based on their artwork
Introduction: Health behaviors are generally regarded as behaviors, which are related to the health status of the individual. Purpose: To evaluate children knowledge and healthy behaviors based on the drawings. Materia...