Tumour Budding and MMP-2 Expression in Breast Invasive Ductal Carcinoma
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 5
Abstract
ABSTRACT Introduction: Breast cancer is a major cause of cancer death among females. Tumour buds are clusters of undifferentiated malignant cells (one cell to less than or equal to five cells) at the invasive front of a tumour. They are believed to be the basis of tumour progression and metastasis. Over expression of MMP-2 {an Extracellular Matrix (ECM) proteolytic enzyme} is considered important for tumour invasion and metastasis. Aim: This study is designed to evaluate tumour budding and expression of MMP-2 in breast invasive ductal carcinoma. Materials and Methods: This cross-sectional observational study was conducted on 61 cases of female breast invasive ductal carcinoma. Cases were obtained from the Department of Pathology, Faculty of Medicine, Cairo University, Giza, Egypt and a private laboratory during the period from June 2015 until September 2016. Tumour budding detection using pan cytokeratin and expression of MMP-2 were evaluated immunohistochemically in 61 cases of female breast invasive ductal carcinoma. MMP-2 expression was evaluated in both neoplastic cells and accompanying stromal component. Results: Significant positive correlations were found between tumour budding and ill defined borders, positive lymph node metastasis and low mitotic count. There were statistically significant positive correlations between expression of MMP-2 in invasive tumour and its expression in both in situ and stromal components. Significant positive correlations were found between expression of MMP-2 in tumour stromal cells and expression of MMP-2 in the in situ component and infiltrated resection margins. When budding was combined with MMP-2 expression in tumour cells; there were significant correlations with Oestrogen Receptor (ER) expression and MMP-2 expression in the in situ component and tumour stroma, and when combined with expression of MMP-2 in tumour stroma; there were significant correlations with expression of MMP-2 in invasive tumour and infiltrated resection margin. Conclusion: Tumour budding might help in assigning subsequent treatment strategies in early breast cancer as in colorectal cancer. MMP-2 expression is seen in the in situ breast cancer and subsequent invasive components and the surrounding stroma, which points to its crucial role in tumour invasiveness and subsequent growth.
Authors and Affiliations
Samia Mohamed Gabal, Amira Mohamed Bassam, Mohamed Emam Sedqi, Rasha Mahmoud Allam
Keywords
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- EP ID EP522530
- DOI 10.7860/JCDR/2018/34941.11540
- Views 68
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