Tumour Necrosis Factor α Gene Polymorphism in Rheumatoid Arthritis and its Association with Oxidative Stress and Dyslipidemia
Journal Title: Journal of Clinical and Diagnostic Research - Year 2019, Vol 13, Issue 6
Abstract
ABSTRACT Introduction: Rheumatoid Arthritis (RA) is a chronic inflammatory disease that affects joints. Increased oxidative stress and inflammation due to elevated levels of Tumour Necrosis Factor-α (TNF-α) leads to pathogenesis of RA. Single Nucleotide Polymorphisms (SNPs) in the promoter region of the TNF gene affects the expression of this pro-inflammatory cytokine TNF-α and TNF-α often leads to development of dyslipidaemia in RA cases. Aim: To investigate the association of SNP, TNFα-308G/A with disease development, plasma TNF-α levels, disease biomarkers, oxidative stress and dyslipidaemia parameters in RA cases. Materials and Methods: In this analytical case-control study, hundred cases of RA diagnosed according to American College of Rheumatology (ACR) Criteria 2010, were recruited from Outpatient Department at Guru Teg Bahadur Hospital, Delhi. Age and sex-matched, 100 apparently healthy individuals were recruited as controls. TNF-α-308G/A polymorphism in cases and controls were screened by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Plasma TNF-α, anti-Cyclic Citrullinated Peptide antibody (anti-CCP), Rheumatoid Factor (RF), and C-Reactive Protein (CRP) were quantified by Enzyme-Linked Immuno Sorbet Assay (ELISA). Biochemical and oxidative stress parameters were measured by standard reference methods. Simple binary logistic regression analysis was done to determine the genetic association of disease with TNF-α-308G/A polymorphism. ANOVA was used for comparative analysis and Pearson’s correlation was applied for correlation of oxidative stress and lipid parameters. The p-value less than 0.05 was considered statistically significant. Results: The present study revealed that TNF-α 308G/A polymorphism was associated with RA (OR=1.9, p=0.02). Significant dyslipidemia, higher MDA and GST levels with decreased GSH and FRAP was observed in RA cases. RA cases with AA genotype presented with significantly higher levels of TNF-α, anti-CCP, CRP, MDA and GST. Plasma levels of TNF-α exhibited a positive correlation with ESR levels (r=0.22, p<0.05) and anti-CCP levels (r=0.21, p<0.05) whereas, MDA was positively correlated with Low-Density Lipoprotein (LDL-C) (r=0.32, p<0.05). Conclusion: The present study establishes that TNF α-308GA polymorphism was positively associated with rheumatoid arthritis and played a crucial role in its development and progression. Screening for genetic polymorphisms can help in identification of the susceptible population, development of personalised treatment and prevention of the occurrence of severe RA.
Authors and Affiliations
Puja Kumari Jha, Mohd Jahid, Rajnish Avasthi, Rafat Sultana Ahmed, Basu Dev Banerjee
Keywords
Related Articles
- EP ID EP605006
- DOI 10.7860/JCDR/2019/41526.12952
- Views 113
- Downloads 0
Early Versus Delayed Intervention with Intracameral Liposomal Amphotericin B in Recalcitrant Keratomycosis: Experience of a Large Case Series
ABSTRACT Introduction: Fungal keratitis has been reported to cause up to 50% of all corneal ulcers in the Indian subcontinent. Fungal keratitis is also associated with a very poor outcome with conventional treatment moda...
Rare Multiple Open Dislocation of Carpometacarpal Joints
ABSTRACT Fracture dislocations of Carpometacarpal (CMC) joints are high-energy injuries, often occurring from vehicle accidents. We here by present a rare case of open dislocation of the second, third, and fourth CMC joi...
Comparative Analysis of Colour Doppler Ultrasonography and CT Angiography in Peripheral Arterial Disease: Studying effects of Vessels Wall Calcification on Diagnostic Evaluation
ABSTRACT Introduction: Peripheral Arterial Disease (PAD) is one of the most common causes of morbidity in diabetes mellitus in Indian population. Aim: To analyse comparative role of CDUS (Colour Doppler Ultrasonography)...
Inguinal Hernia as an Initial Presentation of Pseudomyxoma Peritonei
Pseudomyxoma Peritonei (PMP) is characterized by presence of abundant extracellular mucin in the peritoneum. Preoperative diagnosis of pseudomyxoma peritonei is difficult due to its varied presentation. We report a case...
Short-Term Effect of “Mechanical Diagnosis and Therapy” in the Management of Sacroiliac Joint Pain
ABSTRACT Introduction: Sacroiliac Joint (SIJ) is a familiar nociceptive source of Low Back Pain (LBP). Patients with SIJ pain commonly present with tenderness around the Posterior Superior Iliac Spine (PSIS) which can be...