ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY: A STEP AHEAD TO HPLC
Journal Title: Int J of Pharm Rev& Res - Year 2012, Vol 2, Issue 1
Abstract
UPLC can be regarded as new direction for liquid chromatography. UPLC refers to ultra performance liquid chromatography, improves in three areas: “speed, resolution and sensitivity”. In this system uses fine particles (less than 2.5 μm).So reduces length of column, saves time and reduces solvent consumption. UPLC chromatographic system is designed in a special way to withstand high system back-pressures. Special analytical columns UPLC BEH C18 packed with 1.7 μm particles are used in connection with this system. The quality control analyses of various pharmaceutical formulations are transferred from HPLC to UPLC system. The UPLC system allows shortening analysis time up to nine times and three times comparing to the conventional system using 5 μm and 3 μm particle packed analytical columns respectively. The negative effect of particle size decrease is back-pressure increase about nine times (versus 5 μm) or three times (versus 3 μm), respectively. The separation on UPLC is performed under very high pressures (up to 100 MPa) but it has no negative influence on analytical column or other components of chromatographic system. Separation efficiency remains maintained or is even improved by UPLC
Authors and Affiliations
Tanvi K Desai , Anand A Mahajan , Anil Thaker
Keywords
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