Ultrasound model guided testicular fine needle aspiration in buck (Capra hircus)—An animal model
Journal Title: Asian Pacific Journal of Reproduction - Year 2017, Vol 6, Issue 1
Abstract
Objective: To establish ultrasound guided testicular fine needle aspiration (TFNA) as well as to assess the effectiveness of uni-directional (UD) and multi-directional (MD) TFNA in buck according to testicular cells, echotexture and gross changes of testicle, age of buck was considered. Methods: A total of 120 samples were collected with both directions (UD, n=60) and (MD, n=60) suction from testis of 10 apparently healthy bucks. All slides were stained with May-Grünwald-Giemsa and examined under light microscope with 1 000 × magnifications to count spermatogenic cells, spermatozoa and sertoli cells. The percentage of spermatozoa and sertoli cells were expressed as spermatic index and sertoli cell index. Results: Results revealed no difference in the presence of various spermatogenic and sertoli cells in cell cluster of slides made either unidirectional TFNA or multidirectional TFNA. Early spermatids were the most numerous, followed by late spermatids, primary spermatocytes, spermatogonia. Sertoli cell index was higher in TFNA smears of young bucks prepared 7-13 mo of age and spermatic index was higher in adult bucks 14-24 mo of age. No echogenic change was observed in the echotexture of testisafter TFNA. Conclusions: It seems that TFNA has no serious ill effect on the buck testis when uni-direction aspiration is performed. Moreover, the possibility to standardize this method might provide a greater impulse to the clinical diagnostics of male animal infertility.
Authors and Affiliations
Sattwikesh Paul, Nasrin Sultana Juyena2, Shonkor Kumar Das, Raihana Nasrin Ferdousy, Rahul Deb Sarker, Soheli Jahan Mou
Keywords
Related Articles
- EP ID EP240009
- DOI 10.12980/apjr.6.20170105
- Views 196
- Downloads 0
Effect of graded doses of acetylsalicylic acid on sperm chromatin integrity and maturity of germinal epithelium in adult male mouse
Objective: To evaluate the effect of graded doses of acetylsalicylic acid (ASA) on sperm chromatin integrity and sex hormones in the adult male mouse. Methods: Forty-nine male adult mice were divided into seven groups. C...
Improvement of sexual behavior, sperm quantity and quality by Quercetin in streptozotocin-induced diabetic erectile dysfunction
Objective: To evaluate the effect of Quercetin (QT) on erectile dysfunction and oxidative stress in penile tissue of streptozotocin-induced diabetic rats. Methods: Two weeks after diabetes induction, QT was treated to no...
Improvement of cortical granules migration and in vitro embryo production of vitrified bovine oocyte by 9-cis retinoic acid
Objective: To reveal the effects of 9-cis retinoic acid (RA) on cortical granules (CGs) migration and in vitro embryo production (IVP) rate in meiosis Ⅱ vitrified bovine oocyte. Methods: Following in vitro maturation (IV...
Effect of combination of Gynura procumbens aqueous extract and Trigona spp. honey on fertility and libido of streptozotocin-induced hyperglycaemic male rats
Objective: To study the effects of co-administration of Gynura procumbens (GP) and kelulut honey (KH) on male fertility and libido in diabetes-induced rats. Methods: A total 42 males Sprague Dawley rats aged 8 weeks were...
Effect of preparation program on maternal anxiety of mothers fertilized through in vitro fertilization
Objective: To determine the effect of preparation to play a maternal role in mothers fertilized through in vitro fertilization on maternal anxiety. Methods: A total of 60 mothers undergoing in vitro fertilization were as...