ULTRASTRUCTURAL ORGANIZATION OF APICAL MERISTEMS IN SPRING WHEAT SEEDLINGS UNDER INOCULATION WITH BACTERIA OF AZOSPIRILLUM BRASILENSE 102 NEW STRAIN
Journal Title: Мікробіологія і біотехнологія - Year 2014, Vol 0, Issue 4
Abstract
The aim of study was to investigate the ultrastructural organization of apical meristem cells of spring wheat plants inoculated by diazotrophic bacteria of Azospirillum genus. Methods. The research of endophytic associations formation between A. brasilense 102 and spring wheat plants (Triticum aestivum L. (T. Vulgare Vill)) sort Rannia 93 were carried out under sterile conditions. Electron microscopic study of cells apical meristems was performed in the transmission electronic microscope Jeol JSM 1200-EX. For each variant the calculation was made among 50 samples in 5 stem apex. Results. During the electron microscopic study of shoot apical meristematic cells of spring wheat grown on sterile substrate inoculated with bacteria Azospirillum brasilense 102, we have investigated the ultrastructural organization of izodiametric cells of initiative apex zone. Under the influence of inoculation the pool of meristems increased, the nuclear – cytoplasmic ratio decreased, the size of mitochondria and plastids increased, mitochondria number of meristematic cells, cristae in mitochondria increased. In cells of mesophillous plants inoculated by A. brasilense 102 strain, there were revealed an increase in the number and size of plastids, mitochondria and peroxisomes compared with controls, there were indicating higher level of cellular respiration in the plants inoculated by this strain. Given the inoculation system cristae in mitochondria was more developed, which may indicate high energy activity of organelles in the inoculated plants. Conclusions. Increasing the number and size of the plastids, mitochondria, peroxisomes, as compared to control indicates a high level of cellular respiration in plants, inoculated by A. brasilense 102 strain. The best level of development of cristae in mitochondria may be evidence of high energy activity of organelles of inoculated plants.
Authors and Affiliations
N. I. Adamchuk-Chala, Ye. P. Kopylov
Keywords
Related Articles
- EP ID EP395696
- DOI 10.18524/2307-4663.2014.4(28).48411
- Views 110
- Downloads 0
BIOTECHNOLOGICAL METHODS FOR PROCESSING SUBSTANDARD ORES AND WASTES
The information about using of biotechnological methods for the processing of mineral and technogenic raw materials was analyzed and summarized in the article. A review of the main groups of microorganisms that make up t...
THE EFFICIENCY OF THE COMPLEX USE OF MICROBIAL FORMULATIONS AND FUNGICIDES FOR THE DISEASES CONTROL AND PRODUCTIVITY INCREASE OF VEGETABLE CROPS
The introduction of biotechnologies into the practice of land use, the reduction or replacement of chemicals by bioformulations, including microbial, are relevant for modern crop production. To date, a wide range of biol...
ANTAGONISTIC ACTIVITY OF METABOLIС PRODUCTS OF BACTERIA LACTOBACILLUS PLANTARUM AND ENTEROCOCCUS ITALICUS WITH JOINT ACTION AGAINST PHYTOPATHOGENIC BACTERIA
Aim. Detection of antagonistic activity of metabolic products of Lactobacillus plantarum and Enterococcus italicus ONU547 bacterial strains with joint action against phytopathogenic bacteria in vitro. Methods. Antagonist...
AUXINS BIOSYNTHESIS BY SOIL STREPTOMYCES – THE ANTAGONISTS OF PHYTOPATHOGENIC MICROORGANISMS AND NEMATODES
Aim. Research of the auxins biosynthesis by soil streptomycetes – the antagonists of phytopathogenic microorganismses and plant parasitic nematodes on synthetic and organic nutrient mediums. Methods. Auxins were determin...
SPREADING AND GENOTYPIC STRUCTURE OF ROTAVIRUSES IN THE ODESA REGION
Aim. The study was to determine the distribution and genotypic diversity of rotaviruses in children under the age of 5 years, the circulation of rotavirus and other viral pathogens of intestinal infections in tap water a...