Unifying Equation and Rate Constant for Amylolysis of Glycosidic Bond
Journal Title: Journal of Applied Life Sciences International - Year 2017, Vol 14, Issue 3
Abstract
Objectives: The objectives of this research were mainly to determine the rate of amylolysis of glycosidic bond and to formulate a dimensionally consistent quadratic equation for the determination of the changes in the concentration of substrate, molar concentration of product and the parameter exp (k t) where k and t are the pseudo-first order rate constant and duration of assay respectively. Study Design: Theoretical and Experimental. Place and Duration of Study: Chemistry & Biochemistry Department, Research Division of Ude International Concepts limited (RC: 862217). The research lasted for about 3 months between May and Sep, 2017. Methodology: Bernfeld method of enzyme assay was adopted for the generation of data. Results: The quadratic form of the equation for the quantification of the product of amylolysis gave results that were not significantly different from the use of usual equations (P > 0.05). The rate of hydrolysis of the glycosidic bond was 11851/min and the rate of formation of the product was 23686/min. Conclusion: The quadratic form of the equation for the quantification of the product of amylolysis has a unifying value. It gives results that are similar to that obtainable from the usual equations. The rate of hydrolysis of the glycosidic bond is ~ 1/2nd the rate of formation of the product. The making and breaking of bond seem to be the rate limiting step.
Authors and Affiliations
Ikechukwu Iloh Udema
Keywords
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- EP ID EP318012
- DOI 10.9734/JALSI/2017/36862
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