Use of AFLP in the study of sugarcane rust resistant somaclones
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 4
Abstract
AFLPs (amplified fragment length polymorphism) was carried out from genomic DNA of five rust resistant sugar cane somaclons and their susceptible donor, Saccharum officinarum var B 4362, using three combinations of primers (EcoRI/aca: MseI/acc; EcoRI/aca: MseI/atg and EcoRI/aca: MseI/agg). Six polymorphic bands were obtained, two of these only appeared in the resistant genotypes, which are probably DNA sequences, related to rust resistance locus. These fragments have been cloned to study their nucleotide sequence and to investigate their roll in resistance mechanism develop by these mutants during Puccinia melanocephala infection.
Authors and Affiliations
María Ileana Oloriz, Luis Rojas, Víctor Gil, Milady Mendoza, Ariel Arencibia, Elva Rosa Carmona, Elio Jiménez
Keywords
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