Use of antibiotics to optimize micropropagation of Betula pendula var. carelica Merckl
Journal Title: Бюллетень науки и практики - Year 2018, Vol 4, Issue 5
Abstract
Dependence of the regenerative capacity of single-bud segments of Betula pendula Roth. var. carelica Merckl. shoots on the presence of some antibiotics in a nutrient medium is discussed in the paper. Methods: cell culture in vitro; statistical analysis. Β–lactam antibiotics are optimal at the stage of multiplication of Karelian birch in vitro with the use of single–bud shoot segments as explants. Addition of carbenicillin (500 mg/l) and/or cefotaxime (500 mg/l) to the growth medium allows supporting visually clean tissue culture with no significant negative impact on morphometric parameters and viability of micro–plants.
Authors and Affiliations
I. Kontsevaya
Keywords
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- EP ID EP484137
- DOI 10.5281/zenodo.1246065
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